Table 6.
Multi-response BAP optimization under hypoxia with and without silicone-CaO2.
| Optimization | Optimal level of factors | Response values at the optimum | ||
|---|---|---|---|---|
| Factor A HEMOXCell conc | Factor B Islet density | ATP content per device (RLU) | ATP/LDH ratio (RLU/AU) | |
| w/silicone-CaO2 | 50 µg/mL | 3284 IEQ/device |
8.73 × 106 ≈ 3572 viable IEQa |
9.64 × 106 |
| w/o silicone-CaO2 | 500 µg/mL | 375 IEQ/device |
2.24 × 106 ≈ 656 viable IEQa |
3.83 × 106 |
aNumber of equivalent viable and dead MPIs interpolated from the ATP content standard curve (Supplementary Fig. 3).