TABLE 1.
Primers
| Primer | Sequencea | Gene or 16S RNA (coordinates)b | Application |
|---|---|---|---|
| gyrt3 | ATGGCTGAATTACCTCAATC | gyrA (1–20) | Forward primer for S. aureus and CoNS gyrA |
| gyrt7s | CATCATAGTTATCGATGAAATC | gyrA (454–433) | Reverse primer for CoNS gyrA |
| topot3 | GTGAGTGAAATAATTCAAGATT | grlA (1–22) | Forward primer for S. aureus and S. epidermidis grlA |
| topot3s | CCAGATITTCGTGATGG | grlA (94–110) | Forward primer for CoNS grlA |
| topot7 | TAATAATTAACTGTTTACGTCC | grlA (772–751) | Reverse primer for S. aureus and S. epidermidis grlA |
| topot7s | TCAGCTAAATTATGIGG | grlA (557–541) | Reverse primer for CoNS grlA |
| gcn1 | ATGCGTGAATCATTTTTAGACTATGC | gyrA (53–88) | Forward primer for CoNS gyrA |
| gcn2 | GAGCCAAAGTTACCTTGACC | gyrA (336–317) | Reverse primer for CoNS gyrA |
| tcn1 | AATACGTATGATAAGAATTTCCG | grlA (159–182) | Forward primer for CoNS grlA |
| tcn2 | GTTGTGTCATCATAGTTTGG | grlA (449–430) | Reverse primer for CoNS grlA |
| st1f | GTTAGCGGCGGACGGGTGAG | 16S RNA (95–114) | Forward primer for 16S RNA |
| st2r | CAACATCTCACGACACGAGC | 16S RNA (1096–1077) | Reverse primer for 16S RNA |
| st3r | CGTATTACCGCGGCTGCTGG | 16S RNA (546–526) | Reverse primer for 16S RNA |
a
Sequences are in 5′-to-3′ direction. T3 or T7 promoter tags for “gyr” and “topo” sets are not shown. “I” in topot3s and topot7s = inosine.