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. 2022 Mar 18;13:1466. doi: 10.1038/s41467-022-28788-6

Fig. 2. Growth of breast PDOs in EKGel and BME.

Fig. 2

a–c Brightfield images of PDOs grown in EKGel and BME from PDO-1 (ER+/PR−/HER2−) (a), PDXO-2 (ER−/PR−/HER2−) (b), and PDO-3 (ER+/PR+/HER2−) (c), as in Table 1. Scale bars are 100 µm. d–f Organoids in (a–c) stained for Ki67 (green), human EpCAM (red), and nuclei (blue). Scale bars are 50 µm. g–i Diameters of organoids formed in EKGel (blue) and BME (red) after four passages from PDO-1 (ER+/PR−/HER2−) (g), PDXO-2 (ER−/PR−/HER2−) (h), and PDO-3 (ER+/PR+/HER2−) (i). In (g–i) data shown as mean ± st. dev, with whiskers representing minimum and maximum values, of N = 100 spheroids measured over four repeated experiments. No significant difference between BME and EKGel observed (Student’s t-test, Bonferroni-Dunn method, two tailed, p > 0.01).