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. 2022 Mar 18;12:4695. doi: 10.1038/s41598-022-08467-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Concentration at gel filtration and refold steps in the purification of Q18 IGF-II analogue fusion proteins. Yield is expressed as a percentage of the concentration after dissolution (100%) (data from Fig. 2A presented in B). (B) Table of protein concentration at dissolution (mg/L) and then yield at subsequent steps expressed as a percentage of each of the analogue concentrations at dissolution.