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. 2022 Mar 18;23:15. doi: 10.1186/s10195-022-00633-y

Table 1.

Primer and TaqMan probes used for detection of HSV-1, HSV-2, and CMV

Primer Sequence Amount
HSV forward GAGTGCGAAAA(A/G)ACGTTC 10.0 pmol
HSV reverse GTCTACGAGGACGGCC 10.0 pmol
CMV forward GACACAACACCGTAAAGC 8.0 pmol
CMV reverse CAGCGTTCGTGTTTCC 8.0 pmol
PLZF/X1U GCGATGTGGTCATCATGGTG 1.6 pmol
PLZF/X1L CGTGTCATTGTCGTCTGAGGC 1.6 pmol
TaqMan probe
 HSV-1 CGT CAT CTA CGG GGG TAA GAT GC 4.0 pmol
 HSV-2 TCA TCT GCG GGG GCA AGA T 4.0 pmol
 CMV TCC TCG CAG AAG GAC TCC AG 2.0 pmol
 Extraction control AGC CGG ATC AAG CGT ATG C 1.0 pmol