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. 2022 Feb 28;28:77–86. doi: 10.1016/j.omtn.2022.02.015

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© 2022 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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MBD2 directly binds to CpG islands of promoter of EGR1 and positively activates transcription of them by hypomethylation of promoter

(A) The patterns of CpG islands of EGR1 promoter and five pairs of primer were predicted by the software of MethPrimer 2.0. (B) ChIP assays represents the binding sites of MBD2 interaction with CpG islands of promoter of EGR1. (C) qRT-PCR confirmed the expression of CpG islands of promoter of EGR1. (D) Relative luciferase activity of MBD2 or MBD2 mutation plasmids co-transfected with methylated EGR1 pCpGI plasmid in BUMPT cells. (E) CpG-DNA methylation of the EGR1 promoter region. (F and H) Representative immunoblots of MBD2 and EGR1. (G and I) Densitometry analysis of proteins levels, and normalized to internal control of GAPDH. Data are expressed as means ± SD (n = 6). #p < 0.05 versus scramble or control group. ∗p < 0.05 versus TGF-β1 group.