Figure 2.

ETFα knockdown caused developmental delay and deformities. Tg Flk1: EGFP zebrafish embryos were injected with 5 ng blank control, control morpholino (Ctl-MO), or ETFA target morpholino (ETFA-MO) and were inspected at 8, 24, 32, or 48 hpf, respectively. (a) The mortality was calculated and compared among blank, control MO-treated, and ETFA MO-treated groups (one-way ANNOVA, ^∗P < 0.01, n = 5); (b) developmental status of embryos injected with blank, control MO, and ETFA MO was observed at 8, 24, and 32 hpf, respectively, under a bright field. The photographs in the black box exhibit different deformities of ETFA MO-treated embryos; scale bar = 200 μm; (c) developmental defects were observed at 32 hpf under a confocal microscope. Data presented in (c) were representative fluorescent images of malformation in ETFA-MO-injected embryos at 32 hpf, which include defect sprouting (DS) alone, DS with a short body, DS with spinal curvature, and DS with a curly tail; scale bar = 100 μm; (d) quantitative data of the percentage of malformation in different groups (one-way ANNOVA, ^∗∗∗∗P < 0.00001, n = 3).