Figure 3.

ETFα knockdown caused defective vascular sprouting at 32 hpf. (a) Representative fluorescent images showing different types of vascular defects in ETFA-MO-injected Tg Flk1: EGFP embryos at 32 hpf, including slight (incomplete DLAVs), moderate (short ISVs), and severe (inadequate sprouting); scale bar = 100 μm; (b) quantitative data of the different vascular defects at 32 hpf (one-way ANNOVA, n = 3); (c) the total numbers of intact intersegmental vessels (ISVs) (one-way ANNOVA, ^∗∗∗∗P < 0.00001, n = 15).