Figure 5.

ETFα overexpression rescued vascular defects in ETFA-MO-injected Tg flK1: EGFP embryos. Tg Flk1: EGFP zebrafish embryos were coinjected 5 ng MO with different concentrations of ETFA mRNA as indicated. Embryos were inspected and imaged at 48 hpf. Data presented here were the representative fluorescent images of vascular sprouting (a) and quantitative data of the intact ISVs in different groups (b) (one-way ANNOVA, ^∗P < 0.01, ^∗∗P < 0.001, n = 10); (c) representative immunoblot for ETFα in zebrafish embryos with the indicated treatments. β-Actin was included as the loading control; (d) RT-qPCR analysis of HIF1 gene expression in zebrafish embryos with the indicated treatments. ^∗P < 0.01, ^∗∗∗P < 0.0001, and ^∗∗∗∗P < 0.00001 (one-way ANNOVA, n = 4).