Figure 7.

Ethanol changes the immune response

(A–D) RT-qPCR of (A) Drosomycin, (B) peptidoglycan receptor protein (PGRP) LA, (C) PGRP-SC1A, and (D) PGRP-SC1B. Three biological replicates with three experimental vials each. 6 flies homogenized per replicate. Individual replicates shown. Box shows quartiles; bar is median. Relative-fold expression calculated relative to 0% ethanol bacterially-colonized fly treatment and normalized to ribosomal protein L32 (rpl32). ANOVA with multiple comparisons corrected by Bonferroni method.

(E–H) Treatments were pooled by ethanol concentration to test whether ethanol increases the immune response. Corrected p-values indicated as p < 0.05, ∗; p < 0.01, ∗∗; p < 0.001, ∗∗∗; p < 0.0001. ∗∗∗∗. See also Figure S10.