Figure 1.

Methylation loss following Dnmt3a deletion is time dependent and slow

(A) Schematic representation of the knockout strategy. Exons are represented as filled boxes and loxP sites as red triangles. Tam, tamoxifen.

(B) Schematic representation of the Dnmt3a takeaway experiment. Tam, tamoxifen; WGBS, whole genome bisulfite sequencing.

(C) Table of floxing efficiencies determined by quantitative RT-PCR.

(D) Heatmap of mean methylation values for 33,519 DMRs identified by comparing Dnmt3a^fl/fl − Tam (WT) vs. Dnmt3a^−/− (without dox; null), with mean methylation values for Tam-treated takeaway samples plotted passively. Time of Tam (takeaway) treatment is shown in weeks.

(E) Methylation density of 33,519 DMRs defined in d, in Dnmt3a^fl/fl + Tam. DMR; differentially methylated region, tam; tamoxifen, WGBS; whole genome bisulfite sequencing. Time is shown in weeks. Data are presented as means over all DMRs and all samples from each treatment group: Dnmt3a^−/− (N = 8), Dnmt3a^fl/fl + Tam (N = 6), N = 1 for each takeaway timepoint.