REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
CD11b (MI/70)	Becton Dickinson	565080; RRID: AB_2722548
Gr1 (TB6-8C5)	Biolegend	108456; RRID: AB_2616737
Ter-119 (Ter119)	Becton Dickinson	740875; RRID: AB_2740526
CD71 (C2)	Becton Dickinson	563013; RRID: AB_2737950
B220 (RA3-6B2)	Becton Dickinson	612950; RRID: AB_2870227
CD3e (145-2C11)	Becton Dickinson	564618; RRID: AB_2738868
Sca-1 (D7)	Biolegend	108138; RRID: AB_2564042
c-KIT (2B8)	Becton Dickinson	562609; RRID: AB_11154585
CD34 (RAM34)	Becton Dickinson	553733; RRID: AB_395017
FLT3 (A2F10)	Biolegend	135306; RRID: AB_1877217
CD150 (TC15-12F12.2)	Biolegend	115941; RRID: AB_2629660
CD48 (HM48-1)	Biolegend	103420; RRID: AB_1089037
CD45.1 (A20)	Becton Dickinson	563983; RRID: AB_2738523
CD45.2 (104)	Becton Dickinson	740131; RRID: AB_2739888
Chemicals, peptides, and recombinant proteins
Ammonium Chloride/KCl (ACK) red cell lysis buffer	Sigma	A9434, P9541
SYBR green	Thermo Fisher	K0221
RPMI media	Gibco	11875034
15% FBS	Atlanta Biologicals	S11550H
Tamoxifen	Sigma	T5648
Critical commercial assays
Chromium Single Cell 5′ Kits	10x Genomics	#1000286, #1000263, #1000215
Accel NGS Methyl-Seq DNA library kit	Swift Biosciences	#30096
Deposited data
Aligned Sequence Data	This paper	NCBI: PRJNA797693
Experimental models: Organisms/strains
C57Bl/6-CD45.1 (NCI B6-Ly5.1/Cr)	Charles River Laboratories.	564
C57Bl/6-CD45.2 Dnmt3afl/fl -ERTM-cre mice (B6.Cg-Tg(CAG-cre/Esr1∗)5Amc/J	Jackson Labs	004682
Oligonucleotides
Actin F1 primer: 5’-CGGGCTGTATTCCCCTCCATCG-3’	this paper	N/A
Actin R1 primer: 5’-GCCATGTTCAATGGGGTACTTCAGGG-3’	this paper	N/A
Dnmt3a F1 primer: 5′-CGGTCATTCCAGATGATTCCTC-3′	this paper	N/A
Dnmt3a R1 primer: 5′-TGCTGTGGATGT-AGGAAAGCTG-3′	this paper	N/A
Software and algorithms
WGBS analysis pipeline	https://github.com/genome/analysis-workflows/blob/master/definitions/pipelines/	bisulfite.cwl
RNA-seq analysis pipeline	https://github.com/genome/analysis-workflows/blob/master/definitions/pipelines/	rnaseq.cwl
Metilene	Juhling et al., 2016	v0.2.6; quay.io/biocontainers/metilene:0.2.6--h470a237_1
Partek Flow	Partek, Inc.,	build 10.0.21.0411
CellRanger	10X Genomics	v3.1
Winlist	Verity Software House	version 8
Other
10,000 ppm doxycycline chow	Gateway Lab Supply	1815461-203
EDTA capillary tubes	Sarstetdt	20.1278.100