Fig. 2.

PRMT5 regulates gene expression and ATF4 transcriptional program. a) Transcripts upregulated and downregulated by PRMT5i in OCI-AML-20 as illustrated by RNAseq volcano plots for DMSO control and GSK591 (6 days, 1 μM GSK591). b) Reactome pathways significantly enriched in the PRMT5i downregulated transcript set. c) Significantly enriched transcription factor binding sites in the downregulated gene set as identified by TRRUST (transcriptional regulatory relationships unraveled by sentence-based text-mining) analysis. d) Validation of significantly downregulated transcripts, 3 and 6 days of PRMT5 inhibition. N = 3, mean ± SD shown, *p < 0.05 of the day-matched control sample. Validation was performed in OCI-AML-20 cells using PRMT5 inhibitor LLY283 at 100 nM.