Figure 1.

Binding of Helicobacter pylori and P-fimbriated Escherichia coli to the total nonacid glycosphingolipids of human gastric adenocarcinoma. Thin-layer chromatogram detected with anisaldehyde (A), and autoradiograms obtained by binding of recombinant E. coli strain 291-15 (B), H. pylori strain J99 (C), and H. pylori strain P12 (D). The glycosphingolipids were separated on aluminum-backed silica gel plates, using chloroform/methanol/water 60:35:8 (by volume) as solvent system, and the binding assays were performed as described under the “Experimental procedures” section. The lanes were: lane 1, nonacid glycosphingolipids of human gastric adenocarcinoma, 80 μg; lane 2, reference globotriaosylceramide (Galα4Galβ4Glcβ1Cer), 4 μg; lane 3, reference globotetraosylceramide (GalNAcβ3Galα4Galβ4Glcβ1Cer), 4 μg; lane 4, reference lactotetraosylceramide (Galβ3GlcNAcβ3Galβ4Glcβ1Cer), 4 μg; and lane 5, reference Le^b hexaosylceramide (Fucα2Galβ3(Fucα4)GlcNAcβ3Galβ4Glcβ1Cer), 4 μg. The Roman numbers to the left of the chromatogram in (A) denote the approximate number of carbohydrate units in the bands.