Toxoplasma gondii is not an important contributor to poor reproductive performance of primiparous ewes from southern Australia: a prospective cohort study

Thomas Clune; Amy Lockwood; Serina Hancock; Andrew N Thompson; Mieghan Bruce; Sue Beetson; Angus J Campbell; Elsa Glanville; Daniel Brookes; Colin Trengove; Ryan O’Handley; Caroline Jacobson

doi:10.1186/s12917-022-03211-w

. 2022 Mar 19;18:109. doi: 10.1186/s12917-022-03211-w

Toxoplasma gondii is not an important contributor to poor reproductive performance of primiparous ewes from southern Australia: a prospective cohort study

Thomas Clune ¹, Amy Lockwood ¹, Serina Hancock ¹, Andrew N Thompson ¹, Mieghan Bruce ¹, Sue Beetson ¹, Angus J Campbell ², Elsa Glanville ³, Daniel Brookes ³, Colin Trengove ⁴, Ryan O’Handley ⁴, Caroline Jacobson ^1,^✉

PMCID: PMC8933891 PMID: 35305646

Abstract

Background

Toxoplasma gondii causes reproductive losses in sheep worldwide, including Australia. The reproductive performance of primiparous ewes is typically lower than for mature, multiparous ewes, and younger ewes are more likely to be immunologically naïve and therefore more susceptible to reproductive disease if T. gondii infection occurs during pregnancy. The aim of this study was to assess the impact of infection with T. gondii on the reproductive performance of primiparous ewes in southern Australia using a prospective cohort study. This will inform the need for targeted control strategies for T. gondii in Australian sheep.

Results

Toxoplasma gondii seropositivity using indirect ELISA was detected at 16/28 farms located across southern Australia. Apparent seropositivity to T. gondii was lower in primiparous ewes (1.1, 95% confidence interval (CI) 0.6, 1.8) compared to mature, multiparous ewes (8.1, 95% CI 6.0, 10.5; P < 0.001). Toxoplasma gondii seroconversion during the gestation and lambing period was confirmed for 11/1097 (1.0, 95% CI 0.5, 1.7) of pregnant primiparous ewes that failed to raise a lamb, and 1/161 (0.6, 95% CI 0.1, 2.9) primiparous ewes with confirmed mid-pregnancy abortion.

Conclusions

Low frequency of detection of T. gondii seroconversion during gestation and low frequency of seropositivity to T. gondii suggests that toxoplasmosis was not an important contributor to reproductive losses in primiparous ewes on farms located over a wide geographical area in southern Australia.

Supplementary Information

The online version contains supplementary material available at 10.1186/s12917-022-03211-w.

Keywords: Toxoplasmosis, Lamb survival, Abortion, Lamb mortality, Sheep, Parasite, Reproduction

Background

The number of lambs weaned from primiparous ewes is typically lower and more variable compared to multiparous ewes [1, 2]. However, little research has investigated the causes of foetal and lamb mortality occurring between pregnancy diagnosis in mid-pregnancy and weaning for primiparous ewes. Toxoplasmosis is a globally important disease of sheep that is caused by infection with Toxoplasma gondii and can cause early embryonic deaths, abortions, stillbirths, premature lambs and the birth of weak lambs that have poor survival rates. Zoonotic transmission of T. gondii via ingestion of viable cysts in undercooked sheepmeat is also an important public health issue [3].

Sheep can be infected with T. gondii via ingestion of feed or water contaminated with oocysts that have been shed by a feline definitive host [4]. Vertical transmission after pregnancy-induced recrudescence of persistent infections has also been reported [5, 6]. Reproductive disease is generally observed only following a primary infection in a naïve pregnant ewe, and T. gondii infection usually confers long-lasting protective immunity [7]. Hence, the likelihood of infection and thus immunity increases with age [8]. Young ewes are therefore more likely to be immunologically naïve and susceptible to reproductive disease if exposed to infective oocysts during gestation.

Toxoplasmosis is one of the most commonly diagnosed causes of abortion in ewes in southern Australia [9]. However, the incidence of reproductive disease associated with toxoplasmosis in Australian ewes is not well described. Serological surveys conducted in Australian sheep have demonstrated that T. gondii has a broad geographical distribution with reports of seropositivity on 41–97% of the studied farms and mean individual animal seropositivity ranging from 7 to 62% (Additional file 1). However, most of these studies were restricted to specific regions or do not discriminate between age groups of sheep.

Whilst T. gondii is endemic in Australia, its impact on ewe reproduction, and specifically reproduction for primiparous ewes, is not well quantified. A seroprevalence survey in South Australia reported a negative correlation between within-flock T. gondii seroprevalence and lamb marking rate [10]. However, this study did not investigate seroprevalence between ewe age groups or determine the timing of T. gondii seroconversion relative to reproductive outcome. Similarly, a study in Uruguay identified lower lambing rates for ewes that seroconverted for T. gondii during gestation [11]. Vaccination against T. gondii was associated with increased lamb marking percentages in extensively managed primiparous ewe lambs in New Zealand which suggests that toxoplasmosis was impacting reproduction in flocks that were not vaccinated [12].

The aim of this study was to assess the impact of T. gondii infection on the reproductive performance of primiparous ewes in southern Australia. This will inform the need for targeted control strategies for T. gondii in Australian sheep.

Results

Primiparous ewe reproductive performance

Foetal loss and lamb mortality for progeny of primiparous ewes have been reported in more detail by Clune et al. [13]. Briefly, foetal and lamb mortality between scan 1 and lamb marking was 36% (1567/4351 foetuses; range 14–71%) for primiparous ewe lambs and 28% (582/2103 foetuses; range 20–53%) for primiparous yearlings. Mid-pregnancy abortion was detected in 14/19 primiparous ewe lamb flocks and 6/11 primiparous yearling flocks. In primiparous ewe lamb flocks, mid-pregnancy abortion was detected in 5.2% (155/2968) ewes, ranging 0–50.0% across flocks. In primiparous yearling flocks, mid-pregnancy abortion was detected in 0.8% (16/1886) ewes, ranging 0–4.4% across flocks.

Toxoplasma gondii seropositivity

Apparent and true T. gondii seroprevalence for ewe age categories are shown in Table 1. Apparent T. gondii seroprevalence for primiparous ewes (ewe lambs and yearlings combined) was 1.1% (95% CI 0.6, 1.8). Apparent individual-animal T. gondii seroprevalence was higher for mature ewes compared to primiparous ewe lambs (P < 0.001) and primiparous yearling ewes (P < 0.001). There was no difference in the apparent seroprevalence between primiparous ewes mated as ewe lambs or yearlings (P = 0.214).

Table 1.

Apparent and estimated true seropositivity to T. gondii for primiparous ewes mated as ewe lambs (approximately one-year-old at sampling) or yearlings (approximately two-years-old at sampling) and mature multiparous ewes (aged three-years-old or older) from 28 Australian farms

	Ewes sampled
	Flocks (n)	Individual ewes (n)	Seropositive samples (n)	Apparent seropositivity % (95% CI)	Estimated true seropositivity % (95% CrI)
Primiparous ewes
Ewe lambs	19	839	7	0.8 (0.4, 1.6)^a	0.7 (0.1, 1.6)
Yearling	11	440	7	1.6 (0.7, 3.1)^a	1.6 (0.4, 3.1)
Mature ewes	28	558	45	8.1 (6.0, 10.5)^b	8.1 (5.8, 10.6)

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95% CI: 95% confidence interval

95% CrI: 95% credible interval

^ab Apparent seropositivity proportion (%) with different superscripts are significantly different (two sample proportion z-test (2-tailed) P < 0.05)

Toxoplasma gondii seropositivity was detected in at least one ewe for 16/28 (57%) of the farms. Toxoplasma gondii seropositivity was identified in 12/30 (40%) of primiparous ewe flocks and 11/28 (39%) of mature ewe flocks. For flocks where T. gondii seropositivity was detected, within-flock seroprevalence ranged from 1 to 5% for primiparous ewes (Additional File 2) and 5–50% for mature ewes (Additional File 3). The majority (82%) of seropositive mature ewes were detected on five farms where within-flock seroprevalence for mature ewes ranged 25–50% (Additional File 3).

There was no difference in the proportion of primiparous ewes seropositive to T. gondii between states (Table 2). The proportion of mature ewes that were seropositive to T. gondii was greater for South Australia and Victoria compared to Western Australia (Table 2).

Table 2.

Apparent seropositivity to T. gondii at state-level for primiparous ewes mated as ewe lambs (approximately one-year-old at sampling) or yearlings (approximately two-years old at sampling) and mature multiparous ewes (aged 3 years or older) from 28 Australian farms

State^	Ewe lambs		Yearlings		Mature ewes
State^	Sampled (n)	Seropositive (n (%))	Sampled (n)	Seropositive (n (%))	Sampled (n)	Seropositive (n (%))
WA	338	2 (0.6)^a	160	3 (1.9)^a	200	5 (2.5)^a
SA	221	2 (0.9)^a	160	2 (1.3)^a	178	17 (9.6)^b
VIC	280	3 (1.1)^a	120	2 (1.7)^a	180	23 (12.8)^b

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^SA: South Australia; WA: Western Australia; VIC: Victoria.

^ab Apparent seropositivity proportions (%) within columns with different superscripts are significantly different (two sample proportion z-test (two-tailed) P < 0.05)

Timing of T. gondii seroconversion in primiparous ewes

A total of 1279 primiparous ewes were screened for T. gondii seropositivity (Table 1), of which 1097 were pregnant at scan 1 but failed to rear a lamb to marking (Additional file 2). Of the 1097 ewes that were tested and failed to rear a lamb, mid pregnancy abortion (pregnancy loss between scan 1 and scan 2) was detected in 161 ewes.

Toxoplasma gondii seropositivity was detected for 7 ewes joined as ewe lambs and 7 ewes joined as yearlings (Table 1). Of these, outcome of pregnancy and serial blood samples were available for 12 ewes. The timing of detection of seroconversion in these 12 ewes is shown in Table 3. Seropositivity to T. gondii at mating was detected in 1/12 ewes that were seropositive to T. gondii at lamb marking (or last available sample). Seroconversion to T. gondii after mating was detected for 11/1097 (1.0, 95% CI 0.5, 1.7) of the primiparous ewes that failed to raise a lamb based on detection of seropositivity at lamb marking (or last available sample) but not at mating.

Table 3.

Timing of detection for T. gondii IgG seroconversion using indirect ELISA for primiparous ewes (n = 12) sampled across southern Australia between 2018 and 2020. The earliest detection of seroconversion is bolded

Ewe ID	Farm	Timing of foetal or lamb loss	*T. gondii* seroconversion status
Ewe ID	Farm	Timing of foetal or lamb loss	Pre-mating	Scan 1	Scan 2	Pre-lambing	Lamb marking
Seroconversion detected pre-mating
9463	13	Perinatal death	Positive	Positive	Positive	NA	Positive
Seroconversion first detected during pregnancy
18842	20	Late abortion/ perinatal lamb death	Negative	Positive	Positive	NA	Positive
18857	20	Perinatal death	Negative	Positive	Positive	NA	Positive
3479	3	Late abortion/ perinatal lamb death	Negative	Positive	Negative	Negative	Positive
12114	12	Mid Abortion (scan 2 – scan 3)	Negative	Negative	Positive	NA	NA
13091	7	Late abortion ^a (scan 3 – pre-lambing)	Negative	Negative	Negative	Positive	NA
18121	21	Perinatal death	Negative	Negative	Negative	Positive	Positive
Seroconversion first detected at marking
16527	10	Perinatal death	Negative	Negative	Negative	NA	Positive
16528	10	Perinatal death	Negative	Negative	Negative	NA	Positive
17152	15	Perinatal death	Negative	Negative	Negative	Negative	Positive
18190	22	Perinatal death	Negative	Negative	Negative	Negative	Positive
23364	29	Raised twins	Negative	NA	NA	Negative	Positive

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NA Not available for testing as the ewe was not present for sampling or had been removed from the study flock after abortion was confirmed

^aconfirmed late abortion based on observation of purulent vaginal discharge and opportunistic transabdominal ultrasound at the pre-laambing visit

For the subset of ewes selected for serology that had mid-pregnancy abortion, 1/161 (0.6, 95% CI 0.1, 2.9) ewes had evidence of T. gondii seroconversion after mating evident as T. gondii seropositivity at marking and with no evidence of seropositivity at mating (ewe ID 12114; Table 3). Seropositivity was also detected in a second ewe that aborted but did not have serial blood samples available to determine timing of seroconversion. One ewe that was opportunistically identified with late-pregnancy abortion had evidence of T. gondii seroconversion after mating (ewe ID 13091; Table 3).

Serum samples categorised as negative for T. gondii IgG at a sampling timepoint after detection of T. gondii seroconversion were detected for only one ewe (Ewe 3479; Table 3).

Discussion

Toxoplasmosis was not an important contributor to foetal and lamb mortality between pregnancy scanning and marking for the primiparous ewe flocks in this study. Whilst there was serological evidence of widespread exposure to T. gondii at farm level, seroconversion after mating was evident for only 1% of primiparous ewes that were confirmed to be pregnant and subsequently failed to raise a lamb. Low frequency of T. gondii seropositivity in primiparous ewes was consistent with the absence of detection of T. gondii using qPCR on aborted and stillborn lambs from a subset of farms in this study previously reported by Clune et al. [14] and described in more detail in Additional File 4. These findings are in accord with a recent review of submissions to Australian veterinary laboratories that reported T. gondii was implicated in 5% of sheep abortion investigations, and suggests that toxoplasmosis is a sporadic cause of abortion in Australian sheep [9]. Our findings indicate that routine vaccination for toxoplasmosis is unlikely to be economically justified for many Australian sheep producers unless there is evidence demonstrating high risk of exposure to T. gondii in the specific region.

This study used serial serology to assess the timing of seroconversion relative to abortion or lamb death. Abortions may occur acutely, or up to 8 weeks post-infection, with the outcome of infection being largely dependent on the stage of gestation when T. gondii infection occurred [15, 16]. Toxoplasma gondii IgG antibodies are detectable by P30 ELISA between 3 and 10 weeks after infection [17] and persist for several years [18]. So, it is unlikely that antibodies would have failed to rise to detectable levels or waned to below detectable levels by lamb marking if ewes had become infected with T. gondii during pregnancy and foetal or lamb mortality was related to toxoplasmosis. Based on these assumptions, toxoplasmosis was a plausible aetiology for abortions and lamb mortalities in ewes 12114, 13091, 18842 and 18857 (Table 3). However, attempting to relate maternal serostatus to the occurrence of abortion or lamb mortality can be unreliable due to the variable timeframe between detection of seroconversion and lamb mortality [16]. Toxoplasmosis-associated reproductive disease may be confirmed using foetal serology [16], molecular diagnostics [19], and histopathology [20]. Additionally, quantitative serology to evaluate changes in antibody titres for ewes with suspected toxoplasmosis would allow more accurate interpretation of serial serological results. Nevertheless, even if foetal and lamb mortality for all ewes that were seropositive at lamb marking in this study were due to toxoplasmosis, this still represented a very small contributor to overall foetal and lamb mortality in these flocks.

The unclear association between timing of T. gondii seroconversion and abortion in some ewes was also consistent with observations that other factors were likely to be contributing to abortion and perinatal lamb deaths in these flocks. Abortions, stillbirths and polyarthritis associated with Chlamydia pecorum were detected in primiparous ewe flocks from Western Australia, and non-infectious causes of death (including dystocia and starvation-mismothering) were important contributors to cause of lamb death identified at necropsy [14, 21]. Previous Australian studies have reported dystocia and starvation-mismothering to be important contributors to perinatal lamb mortality [22–24], and sporadic abortion associated with C. pecorum infections in ewe lambs [25]. There was no evidence neosporosis or coxiellosis were contributing to foetal or lamb mortality in the same flocks reported in this study [26, 27]. Abortions associated with Campylobacter fetus fetus were detected by microbial culture from aborted lambs in one flock included in this study [28]. A review of abortion investigations submitted to Australian veterinary laboratories reported campylobacteriosis and listeriosis to be the most common diagnoses made for investigations of ovine abortion or stillbirth [9].

This study reported seropositivity for ewes that were determined to be pregnant by transabdominal ultrasound and subsequently aborted or failed to raise lambs. However, toxoplasmosis can also have impacts on early pregnancy before pregnancy scanning, including embryonic death, resorption and early foetal mortality [29]. Therefore, it is possible that infections occurring early in pregnancy could result in primiparous ewes being not pregnant at scanning. This study did not determine association between T. gondii seropositivity and pregnancy status at scan 1. The low seropositivity reported in this study for primiparous ewes that were pregnant at scan 1 suggests that toxoplasmosis was unlikely to be an important contributor to early reproductive losses on these farms. Further investigation is required to determine if infection in early pregnancy is an important contributor to early pregnancy loss evident as ewes that are not pregnant at scanning.

Toxoplasma gondii seropositivity was detected for 57% of farms in this study, providing further evidence for the parasite’s endemicity and suggesting that exposure to T. gondii occurs on many Australian sheep farms. This was consistent with other Australian studies reporting farm-level seroprevalence ranging 41–97% (Additional file 1). Seropositivity in mature ewes (8.1%) was within the 7–57% range reported for other Australian studies in the last 15 years, and similar to national seroprevalence 11.5% (46/401) for mutton (mature sheep) reported by Hamilton et al in a recent Australian abattoir survey (Additional file 1). Individual-animal T. gondii seropositivity for primiparous ewes in our study (1.1%) was lower than two previous studies reporting seroprevalence of 15 and 17% for Australian slaughter-age lambs in abattoir surveys (Additional file 1). Our study used a commercial indirect ELISA that has good sensitivity and specificity relative to latex agglutination test for sheep sera reported by the manufacturer [30], suggesting that differences in testing methodology are unlikely to explain the difference in seroprevalence between the studies. It is therefore more likely that the low seropositivity in primiparous ewes reflects the sporadic nature of T. gondii infection in Australian sheep rather than differences in serological methods.

Flock and animal seropositivity in mature ewes indicated variable exposure to T. gondii on Australian sheep farms. Most seropositive mature ewes were concentrated on five farms where within-farm seropositivity ranged 25–50%. Variable seropositivity in Australian sheep contrasts to the more consistent exposure reported for sheep farms in New Zealand, United States and United Kingdom [8, 31, 32], and suggests sporadic point source exposure to oocysts, likely via contaminated drinking water or feed source, are the major source of T. gondii exposure on Australian sheep farms. The higher (50%) seropositivity observed for ewes on a farm on Kangaroo Island (Additional file 3) was consistent with previous studies reporting high T. gondii seroprevalence on Kangaroo Island [10, 33]. Interestingly, there was no evidence of seropositivity in the primiparous ewes from the farm on Kangaroo Island, consistent with sporadic exposure to T. gondii. The risk of T. gondii exposure is associated with a range of factors including abundance of the feline definitive host, access to surface water and rainfall [34, 35]. Further work is warranted to determine regional differences in T. gondii exposure and incidence of toxoplasmosis. Improved understanding of regional variation and risk factors for T. gondii exposure on Australian sheep farms would inform cost-benefit analyses for interventions to reduce the risk of toxoplasmosis.

Sampling primiparous ewes was biased towards ewes that were determined to be pregnant and subsequently failed to rear a lamb. If toxoplasmosis was an important contributor to foetal and lamb mortality on these farms, then this sampling bias could result in overestimation of seroprevalence relative to the general population. Notwithstanding this, the low frequency of seropositivity and seroconversions during pregnancy in primiparous ewes that failed to raise lambs does not support the role of T. gondii as an important cause of reproductive losses between scanning and marking on these farms. A case control study would allow for determination of odds ratios for ‘fail to rear’ for ewes that were seropositive for T. gondii. However, due to the very low frequency of seropositivity to T. gondii in these flocks we therefore decided to target ‘fail to rear’ ewes to improve our confidence in the level of seropositivity in this cohort.

One ewe (Ewe 3479; Table 3) was seronegative at two timepoints after testing seropositive at scan 1 then subsequently tested seropositive at marking. The reason for fluctuating serostatus was not determined. However, this could be due to false positive results, issues with test reproducibility, or failure of the test to detect fluctuating titres below the detection limit. There is a lack of published data validating the test against other ‘gold standard’ tests such as microagglutination tests or PCR. A commercial modified agglutination test for T. gondii was validated for Australian sheep [36], but this test is no longer available. The indirect ELISA used in this study was found to have good agreement with modified agglutination test on a subset of sheep sera collected from another study (Additional file 5). Further validation of commercial indirect ELISA for natural T. gondii infections in Australian sheep will inform improved estimation of true prevalence for field studies.

Whilst the observations from this study do not support the need for widespread routine vaccination to reduce foetal and lamb mortality for primiparous ewes in Australia, T. gondii is endemic on Australian farms and associated with sporadic reproductive losses on some farms [9]. The low rates of seropositivity in primiparous and multiparous ewes suggests a lack of protective immunity in a large proportion of ewes [7]. These ewes remain susceptible to reproductive disease if a toxoplasmosis outbreak was to occur during gestation. Control of feral and domestic cat populations on sheep farms and measures to prevent contamination of feed and water sources from cat faeces can reduce the risk of toxoplasmosis outbreaks in susceptible sheep. Vaccination could be warranted in some regions where high farm and individual animal seroprevalence is identified [10], and high incidence of reproductive losses due to T. gondii are confirmed using foetal and lamb necropsy and laboratory investigation. The interpretation of T. gondii serology for the purpose of diagnosing toxoplasmosis is challenging, particularly for field investigations of reproductive loss in extensive sheep production systems where abortions or unusually high incidence of perinatal mortality are challenging to detect at the time losses are occurring. Diagnosis of toxoplasmosis should be supported with detection of T. gondii in tissues where possible, as well as the exclusion of other endemic pathogens.

Conclusion

Toxoplasmosis was not a significant contributor to abortion and perinatal lamb mortality for primiparous ewes on farms across southern Australia. Seropositivity to T. gondii for mature multiparous ewes was detected by indirect ELISA on more than half of the farms included in this study. However, only 1% of primiparous ewes that had confirmed pregnancy and subsequently failed to raise a lamb had evidence of T. gondii seroconversion after mating. Low frequency of T. gondii seropositivity in primiparous ewes during gestation was consistent with the absence of detection of T. gondii using qPCR on aborted and stillborn lambs from a subset of farms.

Methods and study design

Study design, animals and research sites

This cohort study was conducted as part of a larger project investigating reproductive performance of primiparous using 30 primiparous ewe flocks on 28 farms located in Western Australia (n = 11), South Australia (n = 9), and Victoria (n = 10) (Fig. 1) between 2018 and 2020 as previously described [13]. Briefly, farms were located over a wide geographic area that incorporated different rainfall zones (Fig. 1 and Additional file 2). Farms were selected for inclusion based on; having at least 200 primiparous ewes available for the study, capacity to monitor ewes and their progeny over the study period, and with sheep genotype and management that were generally representative of standard commercial sheep farms in the region. Some farms included in the study managed flocks of stud sheep which may increase frequency of monitoring relative to commercial flocks, but the housing (i.e. all flocks were managed in paddocks for the duration of the study) and stocking intensity were broadly comparable to commercial sheep flocks in these regions.

Fig. 1 — Approximate location of farms sampled in Western Australia (A) and South Australia and Victoria (B) adapted from Clune et al. [26]

Each flock was monitored during gestation and lambing. Merino and non-Merino breeds were included in the study (Additional file 2). Non-Merino flocks included Border Leicester, Dorper, White Suffolk and composite breeds. Ewes were mated as either ewe lambs (7–10 months, n = 19 flocks) or primiparous yearlings (18–20 months, n = 11 flocks). Most ewes were naturally mated, and all rams used were confirmed to be negative for Brucella ovis via serology prior to mating. At each farm, twenty mature multiparous ewes aged 3 years or older that had been bred on the same farm were randomly selected for blood sampling only (Additional file 3). All farms ran self-replacing flocks and ewes included in the study were managed according to standard farm practice.

Animal measurements and sample collection

Monitoring of ewes and determination of pregnancy outcome are described by Clune et al. [13]. Briefly, foetal mortality for primiparous ewes was determined based on sequential transabdominal pregnancy ultrasounds (scans). Scan 1 was conducted approximately 85 days (range 62–101) from the start of mating. Scan 2 was conducted at least 30 days after the first scan at approximately 118 days (range 107–136) from the start of mating. The outcome of pregnancy and perinatal lamb mortality were determined based on number (single, twin or triplet) and survival status (lambs dead or alive) for lambs at lambing rounds and at lamb marking (approximately 6 weeks from the start of lambing). Ewe lactation status (lactating or not lactating) was determined by visual observation and/or palpation of the udder at lamb marking. Mid-pregnancy abortion was determined based on loss of pregnancy (i.e., no foetus/es detected) or foetal mortality (i.e., no evidence of foetal viability) between scan 1 and scan 2, and validated using lambing records (i.e. no lamb allocated to ewe at lambing rounds) and udder inspection (i.e. no evidence of lactation) at marking as described by Clune et al. [13]. Ewes that “failed to rear” were determined based on evidence of pregnancy at scan 1 with no live lamb present at lamb marking and no evidence of lactation at lamb marking.

Blood sampling of primiparous ewes was conducted as previously described [26, 27]. Briefly, blood samples for primiparous ewes were collected at five timepoints: pre-mating, scan 1, scan 2, pre-lambing (approximately 140 days from start of mating) and lamb marking (approximately 6 weeks from start of lambing). Blood samples for mature ewes were obtained at a single timepoint during the study period, but timing of sampling relative to lambing and their reproductive outcome was not recorded (Additional File 3). All blood samples were obtained by jugular venepuncture into serum vacutainer tubes with clot activator and stored on ice or at 2 °C. Within 72 h of collection, blood samples were centrifuged at 4000 rpm for 10 min and serum was decanted into 2 mL low protein-binding polypropylene screw cap micro tubes and stored at -20 °C prior to serological testing.

Serology sample selection

The sample size necessary to estimate true prevalence was 239 ewes based on assumed true seroprevalence prevalence of 15% in primiparous ewes, assumed test sensitivity 90%, assumed specificity 99% and desired precision 5%. As 30 flocks were included in the study, this sample size was achieved with at least 8 ewes sampled per flock.

All samples from mature ewes and a sub-sample of at least 40 primiparous ewes from each flock were selected for T. gondii serology as previously described [26, 27]. Briefly, samples for primiparous ewes that were identified as pregnant at the first pregnancy scan but failed to successfully rear a lamb were prioritised for screening. That is, sample selection for primiparous ewes was biased towards ewes that were pregnant and failed to rear a lamb. This included primiparous ewes that were determined to have aborted and ewes for which lamb mortality occurred in the perinatal period. Samples for primiparous ewes that reared lambs were also included for screening where flocks had less than 40 ewes that failed to rear a lamb (Additional file 2). Blood samples collected at lamb marking were used for serology where available. For primiparous ewes where blood samples from marking were not available, blood samples collected at the latest available timepoint were used (i.e. blood sample collected at scan 2 or pre-lambing after foetal mortality was detected).

For primiparous ewes that returned a ‘positive’ result using indirect ELISA at the last time-point, serum samples from earlier timepoints (pre-mating, scan 1, scan 2 and pre-lambing) were tested to determine the timing of seroconversion relative to gestation and foetal or lamb mortality.

Toxoplasma gondii serology

Anti-T. gondii IgG seropositivity was determined using commercial indirect ELISA (ID Screen Toxoplasmosis Indirect Multispecies, ID Vet, France) according to the manufacturer’s instructions [30]. Testing was performed by VETPATH Laboratories (Perth, Western Australia). The results were read at 450 nm using a Multiskan FC, Thermo Scientific spectrophotometer. Positive and negative internal controls were included with each plate. Optical density (OD) values were expressed as the mean percentage of sample/positive (S/P) values, as recommended by the manufacturer: S/P value = (OD_sample − OD_{negative.control})/(OD_{positive control} − OD_{negative.control}). Serum samples were classified as positive (S/P value ≥50), doubtful (S/P value 40 to < 50) or seronegative (S/P value < 40) according to the manufacturer’s recommendation. This assay has a specificity of 100% (95% CI 98.2, 100) and a sensitivity of 100% (95% CI 89.1, 100) using sheep sera validated against latex agglutination test (MAST Group) according to the manufacturer’s internal validation report [30]. A subset of sera from Australian sheep previously tested with a validated modified agglutination test [36] in a separate study were re-tested using the commercial indirect ELISA, with sensitivity 90.5% (95% CI 71.1, 98.3) and specificity 100% (95% CI 64.6, 100) (Additional file 5).

Samples that returned a ‘doubtful’ result (Additional file 6) and a subset of samples that returned a negative result (Additional file 7) were re-tested using an alternate commercial ELISA test (IDEXX Toxotest, IDEXX Laboratories, Switzerland), according to manufacturer’s instructions. Re-testing was performed by Department of Primary Industry and Regional Development Diagnostic Laboratory Service. Each plate included positive and negative controls. The results were read at 450 nm using a Multiskan EX, Thermo Fisher Scientific spectrophotometer. Optical density (OD) values were expressed as the mean percentage of sample/positive (S/P) values, as recommended by the manufacturer: S/P value = (OD_sample − OD_{negative.control})/(OD_{positive control} − OD_{negative.control}). Serum samples were classified as positive (S/P value ≥100), weak positive (S/P value 30 to < 100), suspect (S/P value 20 ≤ 30) or negative (S/P value < 20) according to the manufacturer’s recommendation. This assay has reported specificity 97.5% (95% CI 92.5, 99.4) and a sensitivity 90.9% (95% CI 83.4, 95.6) for sheep [37].

Samples that were ‘doubtful’ for the first test (using ID Screen®) but ‘positive’ or ‘weak positive’ for the second test (using IDEXX Toxotest) were categorised ‘positive’. Results that were ‘doubtful’ for first test and ‘negative’ or ‘suspect’ for second test were considered negative (Additional file 6).

Statistical methods

Lamb mortality was calculated based on the number of lambs alive at marking expressed as a proportion of the number of foetuses identified at scan 1. Lamb mortality was classified as ‘mid-pregnancy abortion’ based on detection of pregnancy loss between scan 1 and scan 2 (and validated with lambing records and ewe lactation status). Mid-pregnancy abortion was expressed as a proportion (%) of ewes with abortion detected between scan 1 and scan 2 relative to the number of ewes that were confirmed pregnant at scan 1.

Apparent T. gondii seropositivity was calculated using the number of ewes categorised as positive expressed a proportion (%) of the ewes tested, with 95% confidence intervals determined using Jeffreys method [38]. Proportion T. gondii seropositivity were compared for the ewe age categories and states using a two-tailed two-sample proportion z-test.

The true seropositivity and 95% credible intervals (95% CrI) were estimated using Bayesian inference, considering the sensitivity and specificity and their 95% CrI derived from manufacturer’s internal validation report [30] as beta-pert distribution for priors [39].

Supplementary Information

Additional file 1.^{(89.5KB, pdf)}

Additional file 2.^{(650.7KB, pdf)}

Additional file 3.^{(532.2KB, pdf)}

Additional file 4.^{(67.8KB, pdf)}

Additional file 5.^{(67.6KB, pdf)}

Additional file 6.^{(567.3KB, pdf)}

Additional file 7.^{(305.2KB, pdf)}

Acknowledgements

We thank the participating farmers who provided access to their animals and facilities, conducted lambing rounds, and collected and stored lambs for necropsy. We thank Celia Smuts, Janine Simmonds and the staff at VetPath Laboratories for their feedback on testing methodology and assistance with the serological testing. We thank Cameron Loomes and the staff at Department of Primary Industry and Regional Development Diagnostic Laboratory Service for their feedback on methodology and assistance with serological testing. We thank Tom La and Nyree Philip (Murdoch University), Louis Lignereux and Rob Paterson (University of Adelaide), Andrew Whale, Mary McQuillan and Patrick Hannemann (Livestock Logic, Hamilton, Victoria), Sean McGrath (Millicent Veterinary Hospital), Simon Edwards and Michelle Smart (Willunga Veterinary Hospital), and Lauryn Stewart and Deb Lehmann (Kangaroo Island Veterinary Hospital) for assistance with sample collection and feedback on the study design. We thank Johann Schroder for helpful feedback on the study design and manuscript.

Abbreviations

CI: Confidence interval
CrI: Credible interval
ELISA: Enzyme-linked immunosorbent assay
qPCR: Quantitative polymerase chain reaction

Authors’ contributions

Conceptualisation: TC, CJ, SH, AT, SB. Methodology: TC, CJ, SB, RO. Formal analysis and investigation: TC, CJ, MB, AL, SH, AC, EG, DB, CT, RO. Writing – original draft preparation: TC, CJ, AL. Writing – review and editing: TC, AL, SH, AT, MB, SB, AC, EG, DB, CT, RO, CJ. Funding acquisition: CJ, SH, AT. Resources: CJ, SH, AT, RO. Supervision: CJ, SH, AT. The author(s) read and approved the final manuscript.

Funding

This project was funded by Meat and Livestock Australia (B.AHE.0318). Meat and Livestock Australia approved the study design and approved the manuscript for publication but was not involved in the collection, analysis or interpretation of data, or in the writing of the manuscript. Molecular diagnostic testing for aborted and stillborn lambs was performed under the Western Australian Ewe Abortion and Newborn Lamb Death Surveillance Program (Department of Primary Industries and Regional Development, Western Australia). Thomas Clune received post-graduate scholarships from Meat and Livestock Australia and Sheep Industry Business Innovation (Department of Primary Industries and Regional Development, Western Australia). Equipment used for this project was funded by the Murdoch University Veterinary Trust.

Availability of data and materials

The datasets generated and/or analysed during the current study not openly available due to privacy of participating enterprises, and are available from the corresponding author on reasonable request. Datasets generated are stored in a controlled access repository by Murdoch University.

Declarations

Ethics approval and consent to participate

All procedures were conducted according to guidelines of the Australian Code of Practice for the Use of Animals for Scientific Purposes and were approved by the Murdoch University Animal Ethics Committee (R3004/17). The owners of sheep included in this study provided informed consent to participate.

Consent for publication

Not applicable.

Competing interests

Angus Campbell is a current Associate Editor for BMC Veterinary Research Journal. None of the authors of this paper have a financial or personal relationship with other people or organisations that could inappropriately influence or bias the content of the paper.

Footnotes

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

References

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4.Innes EA, Bartley PM, Buxton D, Katzer F. Ovine toxoplasmosis. Parasitology. 2009;136(14):1887–1894. doi: 10.1017/S0031182009991636. [DOI] [PubMed] [Google Scholar]
5.Costa FTR, Nogueira DB, Oliveira MAG, Silva SS, Silva RF, Sarmento WF, Azevedo SS, Gennari SM, Pena HFJ, Brasil AWL, et al. Vertical transmission of toxoplasma gondii in naturally infected ewes in the semiarid region of Brazil. Comp Immunol Microbiol Infect Dis. 2021;74:101595. doi: 10.1016/j.cimid.2020.101595. [DOI] [PubMed] [Google Scholar]
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12.Kenyon PR, Pinchbeck GL, Perkins NR, Morris ST, West DM. Identifying factors which maximise the lambing performance of hoggets: a cross sectional study. N Z Vet J. 2004;52(6):371–377. doi: 10.1080/00480169.2004.36454. [DOI] [PubMed] [Google Scholar]
13.Clune T, Lockwood A, Hancock S, Thompson AN, Beetson S, Campbell AJD, Glanville E, Brookes D, Trengove C, O’Handley R, et al. Abortion and lamb mortality between pregnancy scanning and lamb marking for maiden ewes in southern Australia. Animals. 2022;12(1):10. doi: 10.3390/ani12010010. [DOI] [PMC free article] [PubMed] [Google Scholar]
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22.Bruce M, Young JM, Masters DG, Refshauge G, Thompson AN, Kenyon PR, Behrendt R, Lockwood A, Miller DW, Jacobson C. The impact of lamb and ewe mortality associated with dystocia on Australian and New Zealand sheep farms: A systematic review, meta-analysis and bio-economic model. Prev Vet Med. 2021;196:105478. doi: 10.1016/j.prevetmed.2021.105478. [DOI] [PubMed] [Google Scholar]
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24.Hinch GN, Brien F. Lamb survival in Australian flocks: a review. Anim Prod Sci. 2014;54(6):656–666. doi: 10.1071/AN13236. [DOI] [Google Scholar]
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26.Clune T, Lockwood A, Hancock S, Bruce M, Thompson AN, Beetson S, Campbell AJ, Glanville E, Brookes D, Trengove C, et al. Neospora caninum is not an important contributor to poor reproductive performance of primiparous ewes from southern Australia: evidence from a cross-sectional study. Parasitol Res. 2021;120(1):3875–3882. doi: 10.1007/s00436-021-07328-z. [DOI] [PubMed] [Google Scholar]
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Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Supplementary Materials

Additional file 1.^{(89.5KB, pdf)}

Additional file 2.^{(650.7KB, pdf)}

Additional file 3.^{(532.2KB, pdf)}

Additional file 4.^{(67.8KB, pdf)}

Additional file 5.^{(67.6KB, pdf)}

Additional file 6.^{(567.3KB, pdf)}

Additional file 7.^{(305.2KB, pdf)}

Data Availability Statement

[CR1] 1.Kleemann DO, Walker SK. Fertility in south Australian commercial merino flocks: sources of reproductive wastage. Theriogenology. 2005;63(8):2075–2088. doi: 10.1016/j.theriogenology.2004.06.017. [DOI] [PubMed] [Google Scholar]

[CR2] 2.Fowler D. Lamb marking performance for ultrasound scanned ewes in Australian sheep flocks (project AHW.131) North Sydney: Australia MaL; 2007. [Google Scholar]

[CR3] 3.Dubey JP, Murata FHA, Cerqueira-Cézar CK, Kwok OCH, Su C. Economic and public health importance of toxoplasma gondii infections in sheep: 2009–2020. Vet Parasitol. 2020;286(C):109195. doi: 10.1016/j.vetpar.2020.109195. [DOI] [PubMed] [Google Scholar]

[CR4] 4.Innes EA, Bartley PM, Buxton D, Katzer F. Ovine toxoplasmosis. Parasitology. 2009;136(14):1887–1894. doi: 10.1017/S0031182009991636. [DOI] [PubMed] [Google Scholar]

[CR5] 5.Costa FTR, Nogueira DB, Oliveira MAG, Silva SS, Silva RF, Sarmento WF, Azevedo SS, Gennari SM, Pena HFJ, Brasil AWL, et al. Vertical transmission of toxoplasma gondii in naturally infected ewes in the semiarid region of Brazil. Comp Immunol Microbiol Infect Dis. 2021;74:101595. doi: 10.1016/j.cimid.2020.101595. [DOI] [PubMed] [Google Scholar]

[CR6] 6.Williams RH, Morley EK, Hughes JM, Duncanson P, Terry RS, Smith JE, Hide G. High levels of congenital transmission of toxoplasma gondii in longitudinal and cross-sectional studies on sheep farms provides evidence of vertical transmission in ovine hosts. Parasitology. 2005;130(3):301–307. doi: 10.1017/S0031182004006614. [DOI] [PubMed] [Google Scholar]

[CR7] 7.Buxton D, Innes EA. A commercial vaccine for ovine toxoplasmosis. Parasitology. 1995;110(S1):S11–S16. doi: 10.1017/S003118200000144X. [DOI] [PubMed] [Google Scholar]

[CR8] 8.Dubey JP, Kirkbride CA. Enzootic toxoplasmosis in sheep in north-Central United States. J Parasitol. 1989;75(5):673–676. doi: 10.2307/3283047. [DOI] [PubMed] [Google Scholar]

[CR9] 9.Clune T, Beetson S, Besier S, Knowles G, Paskin R, Rawlin G, Suter R, Jacobson C. Ovine abortion and stillbirth investigations in Australia. Aust Vet J. 2021;99(3):72–78. doi: 10.1111/avj.13040. [DOI] [PubMed] [Google Scholar]

[CR10] 10.Lanyon SR, O'Handley RM. Relationship between toxoplasma gondii seroprevalence and lamb marking in south Australian sheep flocks. Aust Vet J. 2020;98(11):525–528. doi: 10.1111/avj.13004. [DOI] [PubMed] [Google Scholar]

[CR11] 11.Savio E, Nieto A. Ovine toxoplasmosis: seroconversion during pregnancy and lamb birth rate in Uruguayan sheep flocks. Vet Parasitol. 1995;60(3):241–247. doi: 10.1016/0304-4017(94)00764-8. [DOI] [PubMed] [Google Scholar]

[CR12] 12.Kenyon PR, Pinchbeck GL, Perkins NR, Morris ST, West DM. Identifying factors which maximise the lambing performance of hoggets: a cross sectional study. N Z Vet J. 2004;52(6):371–377. doi: 10.1080/00480169.2004.36454. [DOI] [PubMed] [Google Scholar]

[CR13] 13.Clune T, Lockwood A, Hancock S, Thompson AN, Beetson S, Campbell AJD, Glanville E, Brookes D, Trengove C, O’Handley R, et al. Abortion and lamb mortality between pregnancy scanning and lamb marking for maiden ewes in southern Australia. Animals. 2022;12(1):10. doi: 10.3390/ani12010010. [DOI] [PMC free article] [PubMed] [Google Scholar]

[CR14] 14.Clune T, Besier S, Hair S, Hancock S, Lockwood A, Thompson A, Jelocnik M, Jacobson C. Chlamydia pecorum detection in aborted and stillborn lambs from Western Australia. Vet Res. 2021;52(1):84. doi: 10.1186/s13567-021-00950-w. [DOI] [PMC free article] [PubMed] [Google Scholar]

[CR15] 15.Castaño P, Fuertes M, Regidor-Cerrillo J, Ferre I, Fernández M, Ferreras MC, Moreno-Gonzalo J, González-Lanza C, Pereira-Bueno J, Katzer F, et al. Experimental ovine toxoplasmosis: influence of the gestational stage on the clinical course, lesion development and parasite distribution. Vet Res. 2016;47(1):43. doi: 10.1186/s13567-016-0327-z. [DOI] [PMC free article] [PubMed] [Google Scholar]

[CR16] 16.Munday BL, Dubey JP. Serology of experimental toxoplasmosis in pregnant ewes and their foetuses. Aust Vet J. 1986;63(11):353–355. doi: 10.1111/j.1751-0813.1986.tb02894.x. [DOI] [PubMed] [Google Scholar]

[CR17] 17.Sager H, Gloor M, Tenter A, Maley S, Hässig M, Gottstein B. Immunodiagnosis of primary toxoplasma gondii infection in sheep by the use of a P30 IgG avidity ELISA. Parasitol Res. 2003;91(2):171–174. doi: 10.1007/s00436-003-0964-9. [DOI] [PubMed] [Google Scholar]

[CR18] 18.Dubey JP, Beattie CP. Toxoplasmosis of animals and man. Boca Raton: CRC Press; 1988. [Google Scholar]

[CR19] 19.Masala G, Porcu R, Madau L, Tanda A, Ibba B, Satta G, Tola S. Survey of ovine and caprine toxoplasmosis by IFAT and PCR assays in Sardinia, Italy. Vet Parasitol. 2003;117(1):15–21. doi: 10.1016/j.vetpar.2003.07.012. [DOI] [PubMed] [Google Scholar]

[CR20] 20.Pereira-Bueno J, Quintanilla-Gozalo A, Pérez-Pérez V, Álvarez-Garcìa G, Collantes-Fernández E, Ortega-Mora LM. Evaluation of ovine abortion associated with toxoplasma gondii in Spain by different diagnostic techniques. Vet Parasitol. 2004;121(1):33–43. doi: 10.1016/j.vetpar.2004.02.004. [DOI] [PubMed] [Google Scholar]

[CR21] 21.Ostfeld N, Islam MM, Jelocnik M, Hilbe M, Sydler T, Hartnack S, Jacobson C, Clune T, Marsh I, Sales N, et al. Chlamydia pecorum-induced arthritis in experimentally-induced and naturally infected sheep. Vet Pathol. 2020;58(2):346–360. doi: 10.1177/0300985820973461. [DOI] [PubMed] [Google Scholar]

[CR22] 22.Bruce M, Young JM, Masters DG, Refshauge G, Thompson AN, Kenyon PR, Behrendt R, Lockwood A, Miller DW, Jacobson C. The impact of lamb and ewe mortality associated with dystocia on Australian and New Zealand sheep farms: A systematic review, meta-analysis and bio-economic model. Prev Vet Med. 2021;196:105478. doi: 10.1016/j.prevetmed.2021.105478. [DOI] [PubMed] [Google Scholar]

[CR23] 23.Jacobson C, Bruce M, Kenyon PR, Lockwood A, Miller D, Refshauge G, Masters DG. A review of dystocia in sheep. Small Rumin Res. 2020;192:106209. doi: 10.1016/j.smallrumres.2020.106209. [DOI] [Google Scholar]

[CR24] 24.Hinch GN, Brien F. Lamb survival in Australian flocks: a review. Anim Prod Sci. 2014;54(6):656–666. doi: 10.1071/AN13236. [DOI] [Google Scholar]

[CR25] 25.Westermann T, Jenkins C, Onizawa E, Gestier S, McNally J, Kirkland P, Zhang J, Bogema D, Manning LK, Walker K, et al. Chlamydia pecorum associated sporadic ovine abortion. Vet Pathol. 2021;58(1):114–122. doi: 10.1177/0300985820967451. [DOI] [PubMed] [Google Scholar]

[CR26] 26.Clune T, Lockwood A, Hancock S, Bruce M, Thompson AN, Beetson S, Campbell AJ, Glanville E, Brookes D, Trengove C, et al. Neospora caninum is not an important contributor to poor reproductive performance of primiparous ewes from southern Australia: evidence from a cross-sectional study. Parasitol Res. 2021;120(1):3875–3882. doi: 10.1007/s00436-021-07328-z. [DOI] [PubMed] [Google Scholar]

[CR27] 27.Clune T, Lockwood A, Hancock S, Thompson AN, Beetson S, Bruce M, Campbell AJ, Glanville E, Brookes D, Trengove C, et al. Coxiella burnetii seroprevalence in primiparous and multiparous ewes from southern Australia: a cross-sectional study. Comp Immunol Microbiol Infect Dis. 2022;80:101727. doi: 10.1016/j.cimid.2021.101727. [DOI] [PubMed] [Google Scholar]

[CR28] 28.Clune T, Lockwood A, Hancock S, Thompson A, Beetson S, Campbell A, et al. On-farm investigation of foetal and lamb losses in maiden ewes. In: Sheep, camelid and goat veterinarians conference (Australian Veternary association): 2021. Wagga Wagga: Australian Veterinary Association; 2021. p. 18–26.

[CR29] 29.Dubey JP. Toxoplasmosis in sheep—the last 20 years. Vet Parasitol. 2009;163(1):1–14. doi: 10.1016/j.vetpar.2009.02.026. [DOI] [PubMed] [Google Scholar]

[CR30] 30.IDvet . France: IDvet, Innovative Diagnostics. 2018. Internal validation report: ID Screen® Toxoplasmosis Indirect Multi-species. [Google Scholar]

[CR31] 31.Dempster RP, Wilkins M, Green RS, de Lisle GW. Serological survey of Toxoplasma gondii and campylobacter fetus fetus in sheep from New Zealand. N Z Vet J. 2011;59(4):155. doi: 10.1080/00480169.2011.579240. [DOI] [PubMed] [Google Scholar]

[CR32] 32.Katzer F, Brülisauer F, Collantes-Fernández E, Bartley PM, Burrells A, Gunn G, Maley SW, Cousens C, Innes EA. Increased Toxoplasma gondii positivity relative to age in 125 Scottish sheep flocks; evidence of frequent acquired infection. Vet Res. 2011;42(1):121. doi: 10.1186/1297-9716-42-121. [DOI] [PMC free article] [PubMed] [Google Scholar]

[CR33] 33.Taggart P, McAllister M, Rutley D, Caraguel C. Oesophageal sarcocystosis observed at slaughter provides a reliable and efficient proximate measure of toxoplasma gondii seroprevalence in sheep. Aust Vet J. 2020;98(7):305–311. doi: 10.1111/avj.12941. [DOI] [PubMed] [Google Scholar]

[CR34] 34.Shapiro K, Bahia-Oliveira L, Dixon B, Dumètre A, de Wit LA, VanWormer E, Villena I. Environmental transmission of toxoplasma gondii: oocysts in water, soil and food. Food Waterborne Parasitol. 2019;15:e00049. doi: 10.1016/j.fawpar.2019.e00049. [DOI] [PMC free article] [PubMed] [Google Scholar]

[CR35] 35.Stelzer S, Basso W, Benavides Silván J, Ortega-Mora LM, Maksimov P, Gethmann J, Conraths FJ, Schares G. Toxoplasma gondii infection and toxoplasmosis in farm animals: risk factors and economic impact. Food Waterborne Parasitol. 2019;15:e00037. doi: 10.1016/j.fawpar.2019.e00037. [DOI] [PMC free article] [PubMed] [Google Scholar]

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PERMALINK

Toxoplasma gondii is not an important contributor to poor reproductive performance of primiparous ewes from southern Australia: a prospective cohort study

Thomas Clune

Amy Lockwood

Serina Hancock

Andrew N Thompson

Mieghan Bruce

Sue Beetson

Angus J Campbell

Elsa Glanville

Daniel Brookes

Colin Trengove

Ryan O’Handley

Caroline Jacobson

Abstract

Background

Results

Conclusions

Supplementary Information

Background

Results

Primiparous ewe reproductive performance

Toxoplasma gondii seropositivity

Table 1.

Table 2.

Timing of T. gondii seroconversion in primiparous ewes

Table 3.

Discussion

Conclusion

Methods and study design

Study design, animals and research sites

Fig. 1.

Animal measurements and sample collection

Serology sample selection

Toxoplasma gondii serology

Statistical methods

Supplementary Information

Acknowledgements

Abbreviations

Authors’ contributions

Funding

Availability of data and materials

Declarations

Ethics approval and consent to participate

Consent for publication

Competing interests

Footnotes

References

Associated Data

Supplementary Materials

Data Availability Statement

ACTIONS

PERMALINK

RESOURCES

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