Fig. 4.

The m⁶A modification of circALG1 promoted CRC metastasis in SW480 cells. A MeRIP assays of m⁶A-modified circALG1 in SW480 cells. B-D Construction of an overexpression plasmid targeting the mutations at m⁶A modification sites of circALG1 (B), qRT-PCR detection of the circALG1 expression level (C), and MeRIP analysis of m⁶A-modification level of circALG1 (D). E Representative images and bar graphs of Transwell migration and invasion assays of cells with different circALG1 m⁶A modification levels. The results are presented as the mean ± s.d. and are representative of at least 3 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ^#p > 0.05