Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Mar 19;21:80. doi: 10.1186/s12943-022-01560-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022, corrected publication 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 6 — PGF was a downstream target of the circALG1/miR-342-5p signalling axis. A Venn diagram of RNA sequencing and downstream target genes of miR-342-5p predicted by TargetScan. B The expression level of PGF in TCGA COAD+READ data. C Prognostic analysis of CRC with low and high PGF expression. D qRT-PCR detection of PGF expression in tumours and adjacent tissues from patients with CRC. E The correlation between PGF expression and miR-342-5p was evaluated by Pearson’s correlation analysis. F The luciferase activities were measured by a dual-luciferase assay, and the Renilla/firefly luciferase light-unit ratio was calculated. G-H Bar graphs of Transwell migration and invasion assays of cells with different PGF expression levels. I WB analyses of total ERK and p-ERK in the MAPK signalling pathway as well as the EMT-related proteins E-cadherin and vimentin in cells with different PGF expression levels. J-K Bar graphs of Transwell migration and invasion assays of cells with PGF inhibition and treatment with the circALG1 overexpression plasmid (top, J) or miR-342-5p inhibitor (bottom, J) and cells with PGF overexpression and treatment with the circALG1 inhibitor (top, K) or miR-342-5p mimics (bottom, K). L WB detection of the expression levels of PGF, total ERK and p-ERK (MAPK signalling pathway) and E-cadherin and vimentin (EMT-related proteins) in cancer and paraneoplastic tissues from patients with CRC. The results are presented as the mean ± s.d. and are representative of at least 3 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ^#p > 0.05