TABLE 1.
Plasmids used in this study
| Plasmid | Relevant characteristicsa | Reference |
|---|---|---|
| pUC19 | 2.69 kbp; Ampr; E. coli cloning vector | 50 |
| pANT841 | 2.75 kbp; pUC19 containing a polylinker with additional cloning sites optimized for cloning DNA with high G+C content; blue-white selectable; Ampr | 17 |
| pANT797 | 8.43 kbp; 1.337-kbp NruI fragment and T4 DNA polymerase-blunted KpnI fragment into PvuII-digested pANT795; E. coli-Streptomyces shuttle vector; expression of genes cloned into polylinker is driven by SnpR-activated snpA promoter; Neor | 41 |
| pANT1400 | 9.05 kbp; 6.3-kbp BamHI fragment from S. griseus subsp. griseus ETH A7796 chromosome into BglII site of pANT841 (contains nonS, nonR, orfB, partial orfC, and region upstream of nonS) | This work |
| pANT1401 | 6.29 kbp; 3.55-kbp SstI fragment from pANT1400 into pANT841 (contains region upstream of nonS) | This work |
| pANT1402 | 5.22 kbp; 2.47-kbp SstI fragment from pANT1400 into pANT841 (contains complete nonS, nonR, and orfB) | This work |
| pANT1403 | 10.93 kbp; 2.50-kbp EcoRI-HindIII fragment from pANT1402 into pANT797 | This work |
a
Neor, neomycin resistance; Ampr, ampicillin resistance; SnpR, small neutral protease regulatory protein; snpA, promoter from Streptomyces sp. strain C5 for the small neutral protease.