Figure 2: NKT17 and γδ T cells receive TCR signals after infection.

A) Immunofluorescence staining of lung tissue in Nur77^GFP reporter mice 14 hours post-infection. CD31 (blue): blood vessels; CD1d-αGalCer-loaded tetramer (purple): iNKT cells; GFP (Green) indicating TCR reporter expression. Top: boxes indicate iNKT cells. Lower left: magnified view of the cells above, including one that is GFP⁺ and one that is not. Lower right: unloaded CD1d tetramer staining. Scale bar: 20 μm. B) Left: representative flow cytometry plot of sub-tissue location of TCR-activated (Nur77^GFP+) lung NKT17 and NKT1 cells at 15 hours after infection. Right: Quantification of GFP⁺ cells of panel A at 15 and 24 hours after infection. N=5–7 mice per group, 3 independent experiments. **p=.0014, ****p<0.0001 (One-way ANOVA with Tukey’s multiple comparisons). C) Left: representative flow cytometry plot of sub-tissue location of TCR-activated (Nur77^GFP+) lung γδ T cells at 15 hours after infection. Right: Quantification of GFP⁺ cells at 15 hours after infection. N= 8–10 mice per group, combined data from 3 independent experiments. ****p<0.0001 (unpaired t test). See also Figure S1.