| Antibodies |
| APC-eF780 anti-TCRβ |
ThermoFisher Scientific |
H57-597, Cat# 47-5961-80, RRID:AB_1272209 |
| BV605 anti-CD8α |
BioLegend |
53-6.7, Cat# 100743, RRID:AB_2561352 |
| BV605 anti-CD19 |
BD Biosciences |
1D3, Cat# 563148, RRID:AB_2732057 |
| AF700 anti-CD4 |
BioLegend |
GK1.5, Cat# 100429, RRID:AB_493698 |
| PE Cy7 anti-ICOS |
BioLegend |
C398.4A, Cat# 313519, RRID:AB_10641839 |
| BV711 anti-CD49a |
BD Biosciences |
HA31/8, Cat# 564863, RRID:AB_2738987 |
| PE anti-GM-CSF |
BioLegend |
MP1-22E9, Cat# 505405, RRID:AB_315381 |
| AF700 anti-IL-17A |
BioLegend |
TC11-18H10.1, Cat# 506914, RRID:AB_536016 |
| PerCP-Cy5.5 anti-IFNγ |
BioLegend |
XMG1.2, Cat# 505821, RRID:AB_961361 |
| BV786 anti-CD45 |
BD Biosciences |
30-F11, Cat# 564225, RRID:AB_2716861 |
| BV421 anti-Siglec-F |
BD Biosciences |
E50-2440, Cat# 562681, RRID:AB_2722581 |
| PE anti-Ly6G |
BioLegend |
1A8, Cat# 127608, RRID:AB_1186099 |
| PerCP-Cy5.5 anti-CD11b |
BD Biosciences |
M1/70, Cat# 561114, RRID:AB_2033995 |
| APC anti-CD11c |
ThermoFisher Scientific |
N418, Cat# 17-0114-81, RRID:AB_469345 |
| BV711 anti-CD103 |
BD Biosciences |
M290, Cat# 564320, RRID:AB_2738743 |
| FITC anti-CD24 |
BD Biosciences |
M1/69, Cat# 553261, RRID:AB_394740 |
| PE Cy7 anti-CD64 |
BioLegend |
X54-5/7.1, Cat# 139314, RRID:AB_2563904 |
| PE anti-CD86 |
BD Biosciences |
GL-1, Cat# 553692, RRID:AB_394994 |
| AF488 anti-IL-23p19 |
ThermoFisher Scientific |
fc23cpg, Cat# 53-7023-82, RRID:AB_2574435 |
| APC anti-IL-1β |
ThermoFisher Scientific |
NJTEN3, Cat# 17-7114-80, RRID:AB_10670739 |
| FITC anti-TCRγδ |
BioLegend |
GL3, Cat# 118105, RRID:AB_313829 |
| anti-mouse CD16/CD32 |
In house purification |
2.4G2 |
| BV421 anti-CD31 |
BioLegend |
390, Cat# 102423, RRID:AB_2562186 |
| AF700 anti-CD45 |
BioLegend |
30-F11, Cat# 103127, RRID:AB_493714 |
| Purified NA/LE Rat IgG2a |
BD Biosciences |
R35–95, Cat# 554687, RRID:AB_479678 |
| Ultra-LEAF purified anti-GM-CSF |
BioLegend |
MP1-22E9 |
| Purified Rat Anti-Mouse IL-2 |
BD Biosciences |
Cat# 554424, RRID:AB_395383 |
| Biotin Rat Anti-Mouse IL-2 |
BD Biosciences |
Cat# 554426, RRID:AB_395384 |
| Bacterial and Virus Strains |
| GFP- Streptococcus pneumoniae URF918 |
Jan-Willem Veening (Kjos et al., 2015) |
|
|
S. pneumoniae serotype 19A isolates |
Fadie T. Coleman, Joseph P. Mizgerd |
|
|
S. pneumoniae serotype 3 strain URF918 |
Japan (Kawakami et al., 2003) |
|
| Chemicals, Peptides, and Recombinant Proteins |
| GolgiStop |
BD Biosciences |
Cat# 554724, RRID:AB_2869012 |
| Bromodeoxyuridine |
BD Biosciences |
Cat# 550891, RRID:AB_2868906 |
| Live dead yellow |
ThermoFisher Scientific |
Cat# L34968
|
| Ghost Dye™ UV 450 Viability Dye |
Tonbo Biosciences |
13-0868-T500 |
| eBioscience™ Permeabilization Buffer (10X) |
ThermoFisher Scientific |
Cat# 00-8333-56 |
| mouse 5-OP-RU-MR1 tetramer |
NIH Tetramer Core |
|
| mouse 6-FP-MR1 tetramer |
NIH Tetramer Core |
|
| α-GalCer-CD1d tetramer |
in-house preparation |
|
| Soluble mouse CD1d |
in-house preparation |
|
| Peroxidase conjugated Streptavidin |
Jackson Immuno Research |
016-030-084 |
| Critical Commercial Assays |
| mouse IL-2 ELISA |
|
|
| Experimental Models: Cell Lines |
| DN3A4-1.2 Va14i NKT cell hybridoma cell line |
M. Bix, University of California, San Fransisco |
|
| Experimental Models: Organisms/Strains |
| B6.129S(C)-Batf3tm1Kmm/J mice |
The Jackson Laboratory |
Cat# JAX:013755, RRID:IMSR_JAX:013755 |
| B6.129S-Csf2tm1Mlg/J mice |
The Jackson Laboratory |
Cat# JAX:026812, RRID:IMSR_JAX:026812 |
| B6.Cg-Tg(Itgax-cre)1-1Reiz/J mice |
The Jackson Laboratory |
Cat# JAX:008068, RRID:IMSR_JAX:008068 |
|
Cd1d1f/f mice |
Birkholz et al., 2015
|
|
|
Traj18−/− mice |
Chandra et al., 2015
|
|
|
Langerin-Cre knockin mice |
Zahner et al., 2011
|
|
| Nur77GFP mice |
Kristin A. Hogquist, University of Minnesota, Minneapolis, Minnesota, USA (Moran et al., 2011) |
|
| C57BL/6J mice |
The Jackson Laboratory |
Cat# JAX:000664, RRID:IMSR_JAX:000664 |
| Software and Algorithms |
| FlowJo software |
Tree Star, Ashland, OR |
|
| Prism 7 and 9 software |
Graphpad Software, San Diego, CA |
|
