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. 2022 Mar 16;10:e13138. doi: 10.7717/peerj.13138

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© 2022 Chen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

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(A) Alizarin red staining and Von Kossa staining of rat aorta. The positively stained regions were analyzed using ImageJ. The scale bar represents 500 um (black) and 200 um (red). (B) Relative expression in RT-qPCR of these hub genes. (C) The expression of hub genes in the thoracic artery were detected by immunohistochemistry and the average optical density values were analyzed by ImageJ. The scale bar represents 500 μm (black) and 100 μm (green).