Table 1.
Determination of transfection efficiency of hASCs transfected with different amounts of lipoplex components
| Transfection assays | Components of transfection complexes (Quantity and ratio) | Transfection Efficiency (mean %eGFP expressing cells ± SD)* | ||||
|---|---|---|---|---|---|---|
| pDNA (µg) | "PLUS" reagent (µL) | Lip-LTX (µL) | Component’s ratio | |||
| 1st assessment | I | 0.5 | 0.5 | 1 | 1 : 1 :2 | 5.58% ± 0.72 |
| II | 0.5 | 0.5 | 1.5 | 1 : 1 : 3 | 6.42% ± 0.95 | |
| III | 0.5 | 0.5 | 2 | 1 : 1 : 4 | 19.42% ± 1.71 | |
| IV | 0.5 | 0.5 | 2.5 | 1 : 1 : 5 | 3.92% ± 1.09 | |
| 2nd assessment | V | 0.5 | 0.5 | 2 | 1 : 1 : 4 | 18.08% ± 1.19 |
| VI | 0.75 | 0.75 | 2 | 1 : 1 : 2.7 | 23.75% ± 1.61 | |
| VII | 1 | 1 | 2 | 1 : 1 : 2 | 8.42% ± 1.11 | |
| VIII | 2 | 2 | 2 | 1 : 1 : 1 | 5.58% ± 0.86 | |
* The results have been presented based on the optimum eGFP expression time point (12 h)