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Optimum condition for the detection of the IFNγ gene
| No. | Phase | Tm (ᵒC) | Time | No. of cycle |
|---|---|---|---|---|
| 1 | Initial Denaturation | 95ᵒC | 1 min | 1 cycle |
| 2 | Denaturation-2 | 95ᵒC | 20 sec | 30 cycle |
| 3 | Annealing | 62ᵒC | 1 min | 30 cycle |
| 4 | Extension-1 | 72ᵒC | 50 sec | 30 cycle |
| 5 | Extension-2 | 72ᵒC | 5 min | 1 cycle |
