Extended Data Table 3.

Primers used to confirm insertion of donor DNA by Sanger sequencing.

Primer ID	Primer Sequence	Primer Description
oKAC227	CCCTAGTGTTTGCAGCCATATCTCC	fwd amplicon primer to sequence Outside of left homology arm for mouse Il3
oKAC242	CCAGGCCAACCATAACTTCGTATAATGTATG	rev amplicon primer overlapping left loxP site to sequence mouse Il3
oKAC229	CTGTACAGTCAGGGTCAAGTTTGTGC	fwd amplicon primer in left homology arm to sequence mouse Il3
oKAC225	GGCGGATCTTGAAGTTCACCTTGATG	rev amplicon primer in EGFP to sequence mouse Il3
oKAC223	GCTGACCCTGAAGTTCATCTGC	fwd amplicon primer in EGFP to sequence mouse Il3
oKAC231	GCTCAGATGATGGTGGTAGTGGATAG	rev amplicon primer outside of right homology arm to sequence mouse Il3
oKAC233	GGACCATGACAGGAACCAGAAGC	fwd amplicon primer to sequence outside of left homology arm for mouse
oKAC240	GGCAAGTGACATGTCCCTATAACTTCGTATAATG	rev amplicon primer overlapping left loxP site to sequence mouse Il3ra
oBK8655	CCCTAAGCTCTTCCCTTCTTGTTGGC	fwd amplicon primer in left homology arm to sequence mouse Il3ra
oBK8670	CCTTCAGAGCCCCACTTCCTGTCGAAG	rev amplicon primer in right homology arm to sequence mouse Il3ra
oKAC224	GACCACATGAAGCAGCACGACTTC	fwd amplicon primer in EGFP to sequence mouse Il3ra
oKAC237	GTTACAACACCTAGAAGTAGTACCTCCTC	rev amplicon primer outside of right homology arm to sequence mouse Il3ra