Extended Data Table 4. Missense mutations detected in CRISPR Cas9 edited mice.
Missense mutations do not influence EGFP signal in Il3 targeted mice but result in quenching of EGFP signaling in Il3rɑ targeted mice. IL-3Rɑ functionality is not impacted (Extended Data Fig. 3i).
| Mouse line | Element of donor in which missense mutation was detected | Missense mutations detected |
|---|---|---|
| Il3GFPfl/fl | Il3 cDNA | D70Y (GAT>TAT) |
| eGFP | E143D (GAG>GAT) | |
| Il3rɑfl/fl | Il3ra cDNA | W295L (TGG>TTG) |
| eGFP | G41C (GGC>TGC), E173Q (GAG>CAG) and A228S (GCC>TCC) |