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. Author manuscript; available in PMC: 2022 Mar 19.
Published in final edited form as: J Cell Biochem. 2021 Apr 13;122(8):827–834. doi: 10.1002/jcb.29919

FIGURE 4.

FIGURE 4

Peptide P1 attenuates Nedd4L-mediated LPA1 degradation and disrupts LPA1/Nedd4L association. (A) MLE12 cells were transfected with LPA1-V5, Nedd4L-V5, P1, or Contp plasmids for 48 h. LPA1-V5, Nedd4L-V5, and β-actin levels were examined by immunoblotting. LPA1-V5 levels were analyzed by ImageJ. n = 3, **p < .01, compared to untreated cells. Shown are representative blots from three independent experiments. (B) MLE12 cells were transfected with P1 or Control peptide (Contp) plasmid for 48 h, and then cell lysates were subjected to immunoprecipitation with a LPA1 antibody, followed by Nedd4L immunoblotting. Input lysates were analyzed by immunoblotting with Nedd4L and β-actin antibodies. Shown are representative blots from two independent experiments. IP, immunoprecipitation; LPA, lysophosphatidic acid receptor