Skip to main content
. Author manuscript; available in PMC: 2023 Apr 1.
Published in final edited form as: J Hepatol. 2021 Dec 23;76(4):921–933. doi: 10.1016/j.jhep.2021.12.014

Figure 4:

Figure 4:

ELK1 regulates proliferative and fibrogenic genes in cholangiocytes. (A) ETS motifs identified by HOMER. (B) ELK1 motif from Jaspar. (C) qRT- PCR for ELK1, FN1, PDGFRB, ACTA2, and PDGFB in NHC transfected with control or ELK1 siRNA (Unpaired Student's t-test *p<0.05,**p<0.01 compared with control siRNA group). (D) WB for ELK1, FN1, PDGFRB, α-SMA and PDGFB in NHC transfected with control or ELK1 siRNA (Unpaired Student's t-test *p<0.05 compared with control siRNA group). (E) WB for p-ELK1, ELK1, p-ERK and ERK in NHC treated with LPS. (F) IF for p-ELK1 in NHC treated with LPS. (G) WB for ELK1, α-SMA and PDGFB in NHC transfected with control or ELK1 siRNA +/− LPS (one-way ANOVA with Bonferroni comparison *p<0.05,**p<0.01). (H) ChIP-qPCR in NHC treated with LPS. (I) WB on p-ELK1 and IgG immunoprecipitates from NHC +/− LPS (Unpaired Student’s t-test *p<0.05 compared with control group).(n=3). Graph bars represent SEM.