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. Author manuscript; available in PMC: 2023 Apr 1.
Published in final edited form as: J Hepatol. 2021 Dec 23;76(4):921–933. doi: 10.1016/j.jhep.2021.12.014

Figure 7:

Figure 7:

ACTA2-AS1 mediates P300- and ELK1-dependent transcription. (A) RIP of ac-CBP/P300-bound ACTA2-AS1 in NHC +/−LPS (Unpaired Student's t-test ****p<0.001 compared with control group). (B, C) NHC transfected with control or ACTA2-AS1 ASO +/− LPS analyzed by ChIP for ac-CBP/P300 and H3K27ac on the PDGFB promoter. (D) WB for PDGFB in cells transfected with ACTA2-AS1 ASO following LPS. (E) WB on ac-CBP/P300, p-ELK1 and IgG immunoprecipitates from HiBEC transfected with ACTA2-AS1 ASO +/− LPS. (F) Cholangiocytes transfected with control or ACTA2-AS1 ASO +/− LPS analyzed by ChIP for ELK1 on the PDGFB promoter. (G) Proliferation of cells transfected with ACTA2-AS1 ASO +/− LPS. (B-D),(F-G) one-way ANOVA with Bonferroni comparison *p<0.05,**p<0.01.(n=3). Graph bars represent SEM.