Extended Data Fig. 11:

Testing the importance of structural interactions for SmrB activity. a, Examples of operons containing both uS21 and SMR-domain proteins. b, The distribution of FLAG-tagged full-length SmrB and a construct with only the SMR domain (residues 88–183) was determined by fractionation over sucrose gradient and detection with an anti-FLAG antibody. A non-specific band is marked with *. c, Northern blots using the 3’-probe against the CRP reporters with the short SecM stalling motif in wild-type cells, bL9-deletion strain (ΔrplI), and a strain where mCherry is fused to the C-terminus of bL9 (bL9-mCherry). Ethidium bromide staining of 16S rRNA serves as a loading control. d, Northern blots using the 3’-probe against the CRP reporters with the short SecM stalling motif in wild-type cells, a strain where MBP is fused to the N-terminus of uS21, and a strain where GFP is fused to the C-terminus of uS6.