Figure 6.

Growth kinetics of the Salmonella Typhimurium SL1344 Δ4PAP strain complemented with mutated versions of AcrA. Abbreviations and concentrations of drugs used: CHL, 0.5 μg mL⁻¹ chloramphenicol; CLI, 8 μg mL⁻¹ clindamycin; DOX, 4 μg mL⁻¹ doxorubicin; EtBr, 16 μg mL⁻¹ ethidium bromide; ERY, 4 μg mL⁻¹ erythromycin; FA, 8 μg mL⁻¹ fusidic acid; MIN, 0.25 μg mL⁻¹ minocycline; NOV, 4 μg mL⁻¹ novobiocin; R6G, 16 μg mL⁻¹ rhodamine 6G. Brackets indicate the preferred channel utilised by the substrate: CH1, channel 1; CH2, channel 2; CH3, channel 3; CH4, channel 4. The data shown are the mean OD₆₀₀ values of three biological replicates. Concentrations of drugs are 0.25× MIC of K366D AcrA.