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. 2022 Mar 19;12:4752. doi: 10.1038/s41598-022-08903-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Accumulation of ethidium bromide in the Salmonella Typhimurium SL1344 Δ4PAP ΔacrB strain complemented with K366D AcrA and AcrB channel 3 (A33W T37W N298W AcrB) mutation. The data shown are the mean of three biological replicates showing maximum RFU values after 30 min of ethidium bromide exposure ± standard deviation. Data were analysed by one-way ANOVA and corrected for multiple comparisons using Tukey’s test. Strains with significantly different ethidium bromide accumulation are indicated with ** (P ≤ 0.01) or **** (P ≤ 0.0001). ns, not significant.