Figure 2.

Lamin B1 is required for the maintenance of LAD. (A) Genome tracks of chromosome 14 (20–107 Mb) display the log2 (ChIP/input) values of lamin A ChIP-seq data, LADs identified by EDD, A (orange, positive PC1 values) and B (blue, negative PC1 values) compartment, gene expression level (FPKM) in WT and lamin B1-KO cells, and gene density. (B) Scatter plot of log2 (ChIP/input) values in WT and lamin B1-KO cells. 2 independent experiments. (C) Distribution of LAD size in WT and lamin B1-KO cells. 2 independent experiments. (D) log2 (ChIP/input) values for ciLAD, fiLAD, fLAD and cLAD genomic regions in WT and lamin B1-KO cells. ***P < 0.001, unpaired t-test