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. Author manuscript; available in PMC: 2022 May 1.
Published in final edited form as: Mayo Clin Proc. 2021 Apr 9;96(5):1122–1127. doi: 10.1016/j.mayocp.2020.12.002

Table 1.

Diagnostic studies supporting laser-capture microdissection assisted liquid chromatography mass spectrometry as the new gold standard for amyloidosis typing

Study, Year Clinic, Country Cohort size Main findings
Vrana, 2009 9 Mayo Clinic, USA 50 cases, 41 validation cohort 98–100% successful identification
Klein, 2011 11 Mayo Clinic, USA 21 cases, nerve tissue 100% identification
Said, 2013 12 Mayo Clinic, USA 147 cases, renal 97% identification
Gilbertson, 2015 13 Mayo Clinic, USA National Amyloidosis Centre, London, UK 142 cases from 38 different organ sites 94% accuracy compared to 76% for IHC. 100% concordance
Mollee, 2016 14 Princess Alexandra Hospital, Brisbane, Australia 138 cases, 35 different organ sites 94% identification rate compared to 39% for IHC. 97% concordance.
Tasaki, 2017 15 Kumamoto University, Japan 186 cases 96% consistent with clinical diagnosis
Park, 2018 16 Samsung Medical Center, Seoul, South Korea 16 cases, compared shotgun with targeted proteomics method for 4 amyloid proteins 68% identification by shotgun proteomics, 100% identification by MRM-MS, 56% for IHC
Aoki, 2018 17 Nippon Medical School, Tokyo, Japan 23 renal cases 91% accuracy. Established 10 glomeruli as minimal requirement
Rezk, 2019 18 National Amyloidosis Centre, London, UK 640 cases including 320 that could not be typed by IHC 85% identification rate. 98% concordance with IHC
Gonzalez Suarez, 2019 19 Mayo Clinic, Rochester, USA 170 cases, renal 100% identification rate compared to 84.6% sensitivity and 92.4% specificity for immunofluorescence
Gonzalez Suarez, 2019 20 Mayo Clinic, Rochester, USA 170 cases, renal 100% identification rate compared to 84.6% sensitivity and 92.4% specificity for immunofluorescence
Abildgaard, 2020 21 Odense Amyloidosis Centre, Odense, Denmark. 106 cases from 6 organs 89.6% accuracy for typing, compared to immunoelectronmicroscopy 91.6%
Dasari, 202022 Mayo Clinic, Rochester, USA 16,175 cases, 31 organs 100% identification rate for 21 different amyloid types, amino acid substitutions identified with 100% specificity in hereditary cases