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. 2022 Mar 19;20:150. doi: 10.1186/s12951-022-01367-z

Table 1.

qPCR primers for key factors of rat chondrocyte cycle

Name Gene name Forward (5′ → 3′) Reverse (5′ → 3′)
Gapdh GAPDH GCATCTTCTTGTGCAGTGCC GATGGTGATGGGTTTCCCGT
Cyclin D1 CCND2 ACCTGTGAGGAAGCCATTCG CCAGCGTGTCCCTTCTCATT
Cyclin D3 CCND3 ACACGCGTCGCTTCTCCTA TGTGACATCTGTGGGAGTGC
CDK4 CDK4 ACCAGGATCTCCCACTAGCA TCAGGTCCCGGTGAACAATG
CDK6 CDK6 GGCCGCAGTAGTCAGTTACC CCAACACTCCAGAGGTCCAC
Cyclin E2 CCNE2 CGCAGTAGCCGTTTACAAGC TCACTGCAAGCACCATCAGT
Cyclin A2 CCNA2 GTCAACCCCGAAAAAGTGGC GCCTTCCATGTGTCTGACCAA
CDK2 CDK2 AGGCGGCAACATTGTTTCAA GACAGGGACTCCAAAGGCTC
Cyclin B1 CCNB1 GGTCGATGTGGAGCAGCATA GGCAAAATGCACCATGTCGT
Cyclin B2 CCNB2 TGGCTGGTCCAAGTCCATTC TGTGCTGCATGACTTCCAGT
CDK1 CDK1 AGGACCAGCTCACAAAAGGG GTGGAAAAGCGGCTTCTTGG
P16 CDKN2A AACACTTTCGGTCGTACCCC CTCCCTCCCTCTGCTAACCT
P21 CDKN1A AAGCCCGAGTTCCTGCTAAC ATCGGCGCTTGGAGTGATAG
P27 CDKN1B GACTCACTCGCGGCTCC TGTTTACGTCTGGCGTCGAA