Table 1.
Number of animals used in different experimental setups
| Methods | Experiments | Subgroups/treatments | Number of animals used |
|---|---|---|---|
| qPCR | Time-dependent changes in the expression of inflammasome-related genes | Intact | 3 |
| Sham | 3 | ||
| 6 h after axotomy | 3 | ||
| 1 day after axotomy | 3 | ||
| 3 days after axotomy | 3 | ||
| 7 days after axotomy | 3 | ||
| 21 days after axotomy | 3 | ||
| Effect of NLRP3 and P2X4 inhibition | Intact | 3 | |
| 1 day after ax. + vehicle | 3 | ||
| 1 day after ax. + 5-BDBD | 3 | ||
| 1 day after ax. + MCC950 | 3 | ||
| ISH | Cellular localization of IL1B mRNA | 1 day after axotomy | 3 |
| 1 day after intraspinal injection of LPS + MDP (positive control) | 1 | ||
| IF | Colocalization of inflammasome components | 1 day after axotomy | 3 |
| 3 days after axotomy | 3 | ||
| 7 days after axotomy | 3 | ||
| Quantification NLRP3 and Iba1/GFAP colocalization | 7 days after ax. + vehicle | 3 | |
| 7 days after ax. + MCC950 | 3 | ||
| Quantification of axonal regeneration | 5 days after ax./coapt. + vehicle | 3 | |
| 5 days after ax./coapt. + MCC950 | 3 | ||
| 5 days after ax./coapt. + intrathecal goat IgG | 4 | ||
| 5 days after ax./coapt. + intrathecal IL-1β neutralization | 4 | ||
| WB | Effect of NLRP3 and P2X4 inhibition | 3 days after ax. + vehicle | 3 |
| 3 days after ax. + 5-BDBD | 3 | ||
| 3 days after ax. + MCC950 | 3 | ||
| IHC | Quantification of microglia and astroglia activation | 3 days after axotomy | 4 |
| 3 days after ax. + vehicle | 4 | ||
| 3 days after ax. + MCC950 | 4 | ||
| 3 days after ax. + intrathecal goat IgG | 4 | ||
| 3 days after ax. + intrathecal IL-1β neutralization | 4 | ||
| 7 days after axotomy | 4 | ||
| 7 days after ax. + vehicle | 4 | ||
| 7 days after ax. + MCC950 | 4 | ||
| 7 days after ax. + intrathecal goat IgG | 4 | ||
| 7 days after ax. + intrathecal IL-1β neutralization | 4 | ||
| SFI and retrograde labeling | Quantification of sciatic nerve regeneration | Axotomy and coaptation | 5 |
| Ax./coapt. + vehicle | 5 | ||
| Ax./coapt. + 5-BDBD | 5 | ||
| Ax./coapt. + MCC950 | 5 | ||
| Total number of animals | 135 | ||
Ax. axotomy, Ax./coapt. axotomy and coaptation, qPCR real-time polymerase chain reaction, ISH in situ hybridization, IF immunofluorescence, WB western blot, IHC immunohistochemistry, SFI sciatic functional index