Table 3.
Primary and secondary antibodies used for western blot (WB), immunohistochemistry (IHC) and immunofluorescence (IF)
| Stainings | Primary antibodies | Secondary antibodies |
|---|---|---|
| IL-1β (WB) | Polyclonal goat against IL-1β, 1:500 (Cat# AF-401-NA, RRID:AB_416684, R&D Systems) | HRP-conjugated rabbit anti-goat IgG (H + L), 1:4000 (Cat# A5420, RRID:AB_258242, Merck-Sigma) |
| β-actin (WB) | Monoclonal mouse against β-actin, 1:10,000 (Cat# A5441, RRID:AB_476744, Merck-Sigma) | HRP-conjugated goat anti-mouse IgG (H + L), 1:4000 (Cat# 115-035-003, RRID: AB_10015289, Jackson ImmunoResearch) |
| Iba1 (IHC) | Polyclonal rabbit against Iba1, 1:500 (Cat# 019-19741, RRID:AB_839504, FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) | Biotinylated goat anti-Rabbit IgG Antibody (H + L), 1:500 (Cat# BA-1000, RRID:AB_2313606, Vector Laboratories) |
| GFAP (IHC) | Polyclonal rabbit against GFAP, 1:500 (Cat# ab16997, RRID:AB_443592, Abcam, Cambridge, UK) | Biotinylated goat anti-rabbit IgG Antibody (H + L), 1:500 (Cat# BA-1000, RRID:AB_2313606, Vector Laboratories) |
| NLRP3 (IF) | Polyclonal goat against NLRP3, 1:100 (Cat# GTX88190, RRID:AB_10723786 GeneTex, Irvine, CA, USA) | Alexa Fluor® 594 Plus Highly Cross-Adsorbed donkey anti-goat IgG (H + L), 1:500 (Cat# A32758, RRID:AB_2762828, Thermo Fisher Scientific) |
| ASC (IF) | Monoclonal mouse against ASC, 1:100 (Cat# sc-271054, RRID:AB_10608960, Santa Cruz Biotechnology, Dallas, TX, USA) | Alexa Fluor® 647 Plus Highly Cross-Adsorbed donkey anti-mouse IgG (H + L), 1:500 (Cat# A32787, RRID:AB_2762830, Thermo Fisher Scientific) |
| Monoclonal rabbit against ASC, 1:100 (Cat# 67-824, RRID:AB_2799736, Cell Signaling Technology, Danvers, MA, USA) | Alexa Fluor® 488 AffiniPure donkey anti-rabbit IgG (H + L), 1:500 (Cat# 711-545-152, RRID:AB_2313584, Jackson ImmunoResearch) | |
| NeuN (IF) | Polyclonal rabbit against NeuN, 1:4000 (Cat# ABN78, RRID:AB_10807945, Merck Millipore) | Alexa Fluor® 488 AffiniPure donkey anti-rabbit IgG (H + L), 1:500 (Cat# 711-545-152, RRID:AB_2313584, Jackson ImmunoResearch) |
| ChAT (IF) | Polyclonal rabbit against ChAT, 1:250 (Cat# GTX113164, RRID:AB_1949973, GeneTex) | Alexa Fluor® 488 AffiniPure donkey anti-rabbit IgG (H + L), 1:500 (Cat# 711-545-152, RRID:AB_2313584, Jackson ImmunoResearch) |
| Polyclonal goat against ChAT, 1:500 (Cat# AB144, RRID:AB_90650, Merck Millipore) | Alexa Fluor® 488 Cross-Absorbed donkey anti-goat IgG (H + L), 1:500 (Cat# A11055, RRID:AB_2534102, Thermo Fisher Scientific) | |
| GFAP (IF) | Polyclonal rabbit against GFAP, 1:500 (Cat# ab16997, RRID:AB_443592, Abcam) | Alexa Fluor® 488 AffiniPure donkey anti-rabbit IgG (H + L), 1:500 (Cat# 711-545-152, RRID:AB_2313584, Jackson ImmunoResearch) |
| Alexa Fluor® 647 AffiniPure donkey anti-rabbit IgG (H + L), 1:500 (Cat# 711-605-152, RRID:AB_2492288, Jackson ImmunoResearch) | ||
| Iba1 (IF) | Polyclonal rabbit against Iba1, 1:500 (Cat# 019–19741, RRID:AB_839504, FUJIFILM Wako Pure Chemical Corporation) | Alexa Fluor® 488 AffiniPure donkey anti-rabbit IgG (H + L), 1:500 (Cat# 711-545-152, RRID:AB_2313584, Jackson ImmunoResearch) |
| TRPV1 (IF) | Polyclonal rabbit against TRPV1, 1:500 (Cat# ACC-030, RRID:AB_2313819, Alomone Labs, Jerusalem, Israel) | Alexa Fluor® 488 AffiniPure donkey anti-rabbit IgG (H + L), 1:500 (Cat# 711-545-152, RRID:AB_2313584, Jackson ImmunoResearch) |
| p75 (IF) | Monoclonal mouse against p75 NGF receptor, 1:500 (Cat# ab6172, RRID:AB_305340, Abcam) | Alexa Fluor® 647 Plus Highly Cross-Adsorbed donkey anti-mouse IgG (H + L), 1:500 (Cat# A32787, RRID:AB_2762830, Thermo Fisher Scientific) |
| NEFM (IF) | Monoclonal recombinant rabbit against NEFM, 1:500 (Cat# MA5-32613, RRID:AB_2809890, Thermo Fisher Scientific) | Alexa Fluor® 488 AffiniPure donkey anti-rabbit IgG (H + L), 1:500 (Cat# 711-545-152, RRID:AB_2313584, Jackson ImmunoResearch) |