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. 2022 Mar 10;63(3):9. doi: 10.1167/iovs.63.3.9

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Figure 1. — Transcriptional characterization of human hyalocytes. (A) Experimental setup: vitreous was collected from five eyes enucleated due to melanoma of the iris, ciliary body or choroid and hyalocytes (CD45⁺, CD11b⁺, CX₃CR1⁺, MatMac⁺, and CCR2⁻) were isolated by Fluorescence-Activated Cell Sorting (FACS) followed by RNA sequencing. (B) Heatmap of the top 30 genes expressed in hyalocytes ordered by mean expression. Each column represents one sample and each row one gene. TPM, transcripts per million. (C) Dotplot visualizing the top 10 gene ontology (GO) biological processes, which the top 30 genes expressed in hyalocytes were involved in. The size of the dots represents the number of associated genes (count). The adjusted P value of each GO term is shown by color. (D, E) Morphologic characterization of human hyalocytes obtained from the vitreous of a donor eye (enucleated due to ciliary body melanoma) using immunohistochemical staining against IBA1 (red), phalloidin (green), and DAPI (blue), including an overview of several hyalocytes (D) and a more detailed view of one hyalocyte (E).