Table 2.
Comprehensive mutagenesis of human LSP
| WT base1,2 | Base substitutions | Transcription efficiency3, % of WT | Notes |
|---|---|---|---|
| +4A (403) | T | 19+/-12 | |
| G | 38+/-8 | ||
| C | 22+/-12 | ||
| +3G (404) | T | 28+/-4 | |
| C | 13+/-2 | ||
| A | <2 | ||
| +2A (405) | T | <2 | |
| G | 42+/-15 | ||
| C | 24+/-10 | ||
| +1A (406) | T | <2 | Compared to the 19 nt product in WT |
| G | 100+/-12 | Compared to the 19 nt product in WT | |
| C | <2 | Compared to the 19 nt product in WT | |
| -1A (407) | T | 44+/-16 | |
| G | <2 | ||
| C | 20+/-5 | ||
| -2A (408) | T | 95+/-22 | |
| G | 75+/-30 | ||
| C | 20+/-6 | Enhanced transcript slippage | |
| -3C (409) | T | 5+/-2 | Enhanced transcript slippage |
| G | <2 | ||
| A | <2 | ||
| -4C (410) | T | <2 | |
| G | <2 | ||
| A | <2 | ||
| -5G (411) | T | 100 +/-11 | |
| C | 59+/-36 | ||
| A | 11+/-1 | ||
| -6C (412) | T | 92+/-4 | |
| G | 42+/-6 | ||
| A | 92+/-10 | ||
| -7C (413) | T | 54+/-18 | |
| G | 34+/-12 | ||
| A | 55+/-9 | ||
| -8A (414) | T | 59+/-8 | |
| G | 102+/-17 | ||
| C | 80+/-33 | ||
| -9T (415) | G | 48+/-5 | |
| C | 71+/-3 | ||
| A | 51+/-5 | ||
| -10A (416) | T | 105+/-3 | |
| G | 97+/-7 | ||
| C | 98+/-3 |
1In the non-template strand of the LSP promoter.
2Position in human mtDNA is given in parenthesis.
3Assayed by the run-off transcription assay using all four NTPs and measured for the 19 and 20 nt long RNA products.