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. 2022 Feb 21;50(5):2736–2753. doi: 10.1093/nar/gkac068

Figure 1.

Figure 1.

Quantification of full-length and reinitiated peptides in incorporation of consecutive Pro residues. (A) mRNA and expressed peptide sequences: full-length peptide (FLP1, FLPS1, FLPS2 and FLP2), drop-off peptide (DoP2), and reinitiated peptide (RiP2). (B) Titration of EF-G in translation of mRNA1, mRNAS1, mRNAS2 and mRNA2 in the absence or presence of 5 μM EF-P. Expressed peptides were labelled by [14C]-Asp and quantified by autoradiography. Expression levels of FLP1, FLPS1, FLPS2, FLP2, RiPS2 and RiP2 are shown. The ratio of FLPS2 or FLP2 was defined as (the yield of FLPS2 or FLP2)/(the sum of yields of translation products). Since only FLPs were observed in translation of mRNA1 and mRNAS1, ratio of them were omitted. n = 3. (C) Titration of RF2, RF3 and RRF in translation of mRNA2 in the absence of EF-P. Yields of FLP2 and RiP2 were quantified by autoradiography. n = 3.