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. 2022 Feb 21;50(5):2736–2753. doi: 10.1093/nar/gkac068

Figure 2.

Figure 2.

Mass spectrometric analysis of full-length and truncated peptides in incorporation of consecutive Pro. (A) Identification of translation products by MALDI-TOF MS. Peptides were expressed with 0.03, 0.26 or 3.0 μM EF-G in the absence of EF-P. *: Na+ or K+ adduct peaks, †: Unidentified peaks. ‘Calc.’ and ‘Obs.’ indicate calculated and observed m/z values, respectively. (B) MALDI-TOF MS/MS identification of RiP2 generated in the translation of mRNA2 with 0.26 μM EF-G. (C) Identification of translation products expressed in the presence of 5 μM EF-P by MALDI-TOF MS. (D) LC-ESI MS identification of drop-off DoP2-tRNAPro derivatives treated with PTH and/or RNase A. Their extracted ion current chromatograms (XICs) were extracted by using the indicated m/z values. Concentrations of EF-P, PTH and RNase A were 5 μM, 1 μM and 50 μg/ml, respectively. Black arrow-heads indicate the peaks of DoP2-Adenosine, and a green arrow-head DoP2. (E) Deconvoluted LC-ESI MS/MS spectra of DoP2-Adenosine observed at RT = 5.74 min (2.1) and RT = 6.16 min (2.2) in the XIC 2 in Figure 2D.