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. 2022 Feb 21;50(5):2736–2753. doi: 10.1093/nar/gkac068

Figure 7.

Figure 7.

LC-ESI MS analysis of N-terminal fragments of YhhM-fh expressed in E. coli. (A) N-terminal peptide sequences generated by Lys-N digestion of full-length YhhM-fh (FLP) and reinitiated YhhM-fh lacking its N-terminal amino acids (RiPΔN1, RiPΔN2 and RiPΔN3). MOx: Methionine oxidized on its sidechain thioether. (B) Full-range (left) and magnified (right) XICs of N-terminal peptides generated by Lys-N digestion of YhhM-fh expressed alone (red) and co-expressed with EF-P (blue). XICs were extracted on the observed m/z values (Obs.). (C, D) LC-ESI MS quantification of ion response (C) and ratio (D) of N-terminal fragments generated by Lys-N digestion of YhhM-fh expressed in the absence or presence of EF-P. Ratios were calculated as (ion response of an N-terminal fragment)/(sum of ion responses of all the N-terminal fragments). SD of three independent experiments using YhhM-fh expressed without EF-P and SD of four independent experiments using YhhM-fh expressed with EF-P are shown. *P < 0.1 in Welch's t test. **P < 0.05 in Welch's t test.