Table 1.
Summary of anti-CRISPR proteins reported in this study and their inhibitory activities
| Acr | Strain | Accession number | Bacterial Assays | Human Assays | ||||
|---|---|---|---|---|---|---|---|---|
| SpyCas9 | St1Cas9 | St3Cas9 | SpyCas9 | St1Cas9 | St3Cas9 | |||
| AcrIIA24 | Streptococcus phage CHPC930 | AZF92405.1 | + | - | +++ | - | ND | +++ |
| AcrIIA25 | Streptococcus phage P7602 | ARU14017.1 | ++ | - | +++ | + | ND | ++ |
| AcrIIA25.1 | Streptococcus sp. F0441 | WP_009730541.1 | +++ | - | + | +++ | ND | ND |
| AcrIIA26 | Streptococcus sp. isolate bin_32 | RKW07596.1 | +++ | - | - | +++ | ND | ND |
| AcrIIA27 | Streptococcus pyogenes K23866 | WP_003055855.1 | +++ | - | +++ | +++ | ND | +++ |
| AcrIIA28 | Streptococcus phage Javan128 | QBX23430.1 | +++ | - | +++ | +++ | ND | +++ |
| AcrIIA29 | Streptococcus pyogenes NS3335 | WP_136281566.1 | - | - | ++ | ND | ND | + |
| AcrIIA30 | Streptococcus gordonii NCTC7870 | WP_143877237.1 | - | +++ | - | ND | ++ | ND |
| AcrIIA31 | Streptococcus sp. SR1 | WP_033585134.1 | - | +++ | - | ND | +++ | - |
| AcrIIA32 | Streptococcus uberis NZ01 | WP_199763731.1 | +++ | - | +++ | +++ | ND | +++ |
| AcrIIA32.1 | Streptococcus equinus AR3 | WP_094140900.1 | +++ | - | +++ | +++ | ND | +++ |
20% < inhibitory activity < 50%, marks “+”; 50% < inhibitory activity < 80%, marks “++”; and inhibitory activity > 80% marks “+++”. ND, not determined.