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. 2022 Feb 22;50(5):e26. doi: 10.1093/nar/gkac108

Figure 4.

Figure 4.

dPguCas13b repressed the usage of alternative terminal exon PAS. (A) Illustration of genes containing alternative terminal exon PASs. The positions of gRNA target regions were indicated. (B) UCSC genome browser tracks for RNA-Seq and 3′end mRNA Seq were shown for the human gene eIF4E2. The alternative terminal exon PAS with the dominant usage (alternative PAS) and the distal PAS (disPAS) were indicated. (C) The usage of the alternative PAS in gene eIF4E2 was repressed after transfection of 3xEGFP-dPguCas13b together with gRNAs targeting the PAS signal (gPAS) and 3′ splicing site (g3′SS). (D) UCSC genome browser tracks for RNA-Seq and 3′end mRNA Seq were shown for the human gene SEPTIN8. The alternative terminal exon PAS with the dominant usage (alternative PAS) and the distal PAS (disPAS) were indicated. (E) The usage of the alternative PAS in gene SEPTIN8was repressed after transfection of 3xEGFP-dPguCas13b together with gRNAs targeting the PAS signal (gPAS) and 3′ splicing site (g3′SS). For each experiment, three independent repeats were performed. Error bars represent SEM. ****P < 0.0001, paired two-way Student's t-test.