Figure 10.

Frequencies and physical properties of non-canonical transposon insertion sites generated by the H187V mutant of the Sleeping Beauty transposase. (A) Relative frequencies of dinucleotides occurring at non-TA sites by the H187V mutant (bars) and the relative base stacking energies (red) and protein-inducible twist (blue) associated with each dinucleotide. (B) SeqLogo analysis of non-canonical insertion sites. (C) DNA flexibility as measured by stacking energy for canonical versus non-canonical insertion sites. Averages flexibilities were calculated from di-nucleotide values applied to all the non-TA insertion sites of the H187V mutant in 60-nucleotide-long windows centered around the insertion (black triangle).