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. 2022 Feb 21;50(5):2807–2825. doi: 10.1093/nar/gkac092

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© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Transposition activities of Sleeping Beauty transposase mutants. Relative transposition efficiencies for H187 (A), P247 (B) and K248 [reproduced from (55)] (C). Plasmids expressing transposase mutants were transiently cotransfected with a transposon-donor plasmid (pT2B/puro) into HeLa cells. Cells were selected for puromycin resistance and stained with methylene blue to identify viable cell colonies. Colony numbers were normalized to the SB100X positive control, whose transposition efficiency was set to 100%. An inactive SB transposase (D3) was included as negative control. Data are represented as mean ± SD, n = 3 biological replicates. Differences in transposition activities are significant as determined by Student's t-test for the indicated mutants. Transpositional differences for all of the other mutants were not supported by statistical significance.