Figure 7.

Right ventricular (RV) mitochondrial remodeling in response to pulmonary artery banding (PAB) is dependent on sex and ovariectomy (OVX). B–D: effects of sex and OVX on mitochondrial content (B), mitochondrial size (C), and gene expression (fold change in delta cycle threshold values, or ddCT) of peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α; D) are shown. A: representative electron micrographs captured at ×25,000 magnification are shown for each group. Number of mitochondria was calculated as the counted number of mitochondria per high-power field (HPF). Mitochondrial size was calculated as the cross-sectional area (CSA) of mitochondria per HPF. Note the significant decrease in PGC-1α expression in OVX Sham rats. n = 8–10 animals per group. Values are presented as means ± SE. P < 0.05 for *PAB vs. respective Sham and ‡OVX vs. intact female (2-way ANOVA with Tukey’s honestly significant difference multiple comparison correction).