Figure 4.

Acute exercise rapidly increases mitochondrial mitophagy proteins. Protein content for mitophagy proteins Parkin (A), BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 (BNIP3) (B), mitofusin-2 (Mfn2) (C), and dynamin-1-like protein (Drp1) (D), as well as citrate synthase (E) were examined in mitochondria isolates from wild-type (WT) female mice 0- and 2-h post-condition. Quantified proteins (F) were normalized to total protein using amido black staining for quantitation. T, P < 0.05 main effect for time (2 h vs. 0 h); E × T, P < 0.05 exercise by time interaction; x, P < 0.05 within condition exercise effect (vs. sedentary, SED); t, P < 0.05 within condition time effect (vs. 0 h).