Figure 1.

Generation of podocyte-specific transcriptional coactivator with PDZ-binding motif (Taz) knockout mice. A: scheme of generation of Taz^podKO and Taz^podWT mice. B: immunoblot analysis of isolated glomerular lysates indicated reduced TAZ but upregulated Yes-associated protein (YAP) expression. C: immunofluorescence staining showed that TAZ was effectively deleted but YAP was upregulated in Taz^podKO mouse podocytes (red: nephrin, a podocyte marker; green: TAZ or YAP). Original magnification: ×600. D: immunoblot analysis of nuclear and cytosolic fractions of isolated glomeruli confirmed that both TAZ and YAP are primarily confined to the nuclei of glomerular cells, and both cytoplasmic and nuclear YAP were upregulated in Taz^podKO mice. ***P < 0.001, **P < 0.01. WT, wild type.