Figure 5.

Transcriptional coactivator with PDZ-binding motif (TAZ) regulates expression of synaptopodin (Synop), zonula occludens (ZO)-1 (Tjp1), and ZO-2 (Tjp2) in podocytes. A: screening of actin cytoskeleton regulators by quantitative PCR revealed that knockdown of TAZ significantly inhibited synaptopodin, ZO-1, and ZO-2 gene expression in mouse primary podocytes. B: expression of synaptopodin, ZO-1, and ZO-2 were slightly increased by knockdown of large tumor suppressor 1/2 (Lats1/2) with siRNA transfection and was not affected by overexpression of an active Yes-associated protein (YAP) mutant. C: infection of Cre adenovirus in cultured podocytes from immortalized^Taz mice inhibited mRNA transcription of synaptopodin, ZO-1, and ZO-2. D: infection of Cre adenovirus in cultured podocytes from immortalized^Taz mice inhibited protein expression of synaptopodin, ZO-1, and ZO-2. E: chromatin immunoprecipitation assay indicated that both TEAD and TAZ bind to the promoter of Tjp1, Tjp2, and Synop genes. F: inhibition of synaptopodin, ZO-1, and ZO-2 expression was confirmed in isolated glomerular lysates from Taz^podWT or Taz^podKO mice. ***P < 0.001, **P< 0.01, *P < 0.05. Cd2AP, CD2-associated protein; Ctrl, control; hYAP, human YAP; WT, wild type.