Figure 5.

Expression and localization of proteins of interest within the PND4 ovary. The in situ immunofluorescent expression of three proteins of interest (A-C) were explored in the neonatal mouse ovaries and were co-localized with a nuclear marker (DAPI, blue), a granulosa cell marker (GATA4 or FOXL2, green), and either (A) ZFX (B) POD1 or (C) FRZB in red. Representative images from PND4 selected as they include populations of primordial, activating and primary follicles. Representative images are indicative of n = 4–6 biological replicates of both PND1 and PND4 performed in triplicate. Images taken at 60× magnification, scale bars represent 20 μm with dotted circles outlining primordial follicles, solid lines outlining activating or primary follicles. Arrows indicate extracellular staining regions; asterisks indicate activating granulosa cells.